Abstract
The inhibition of substrate and product on the growth of Klebsiella pneumoniae in anaerobic and aerobic batch fermentation for the production of 1,3-propanediol was studied. The cells under anaerobic conditions had a higher maximum specific growth rate of 0.19 h−1 and lower tolerance to 110 g glycerol l−1, compared to the maximum specific growth rate of 0.17 h−1 and tolerance to 133 g glycerol l−1 under aerobic conditions. Acetate was the main inhibitory metabolite during the fermentation under anaerobic conditions, with lactate and ethanol the next most inhibitory. The critical concentrations of acetate, lactate and ethanol were assessed to be 15, 19, 26 g l−1, respectively. However, cells grown under aerobic conditions were more resistant to acetate and lactate but less resistant to ethanol. The critical concentrations of acetate, lactate and ethanol were assessed to be 24, 26, and 17 g l−1, respectively
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H Biebl K Menzel AP Zeng WD Deckwer (1999) ArticleTitleMicrobial production of 1,3-propanediol Appl. Microbiol. Biotechnol. 52 289–297
H Biebl AP Zeng K Menzel WD Deckwer (1998) ArticleTitleFermentation of glycerol to 1,3-propanediol and 2,3-butanediol by Klebsiella pneumoniae Appl. Microbiol. Biotechnol. 50 24–29
KK Cheng DH Liu Y Sun (2004) ArticleTitle1,3-Propanediol production by Klebsiella pneumoniae under different aeration strategies Biotechnol. Lett. 26 911–915
O Fond NB Jansen GT Tsao (1985) ArticleTitleA model of acetate and 2,3-butanediol inhibition of the growth and metabolism of Klebsiella oxytoca Biotechnol. Lett. 7 727–732
H Huang CS Gong GT Tsao (2002) ArticleTitleProduction of 1,3-propanediol by Klebsiella pneumoniae Appl. Biochem. Biotechnol. 98 687–698
K Menzel K Ahrens AP Zeng WD Deckwer (1998) ArticleTitleKinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: IV Enzymes and fluxes of pyruvate metabolism. Biotechnol. Bioeng. 60 617–626
AP Zeng H Biebl H Schlieker WD Deckwer (1993) ArticleTitlePathway analysis of glycerol fermentation by Klebsiella pneumoniae: regulation of reducing equivalent balance and product formation Enzyme Microb. Technol. 9 770–779
AP Zeng A Ross H Bieble C Tag (1994) ArticleTitleMultiple product inhibition and growth modeling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation Biotechnol. Bioeng. 44 902–911
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Revisions requested 8 september; Revisions received 2 November 2004
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Cheng, KK., Liu, HJ. & Liu, DH. Multiple growth inhibition of Klebsiella pneumoniae in 1,3-propanediol fermentation. Biotechnol Lett 27, 19–22 (2005). https://doi.org/10.1007/s10529-004-6308-8
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DOI: https://doi.org/10.1007/s10529-004-6308-8