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Integrated analysis of miRNAs and their targets reveals that miR319c/TCP2 regulates apical bud burst in tea plant (Camellia sinensis)

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Abstract

Main conclusion

The roles of microRNA-mediated epigenetic regulation were highlighted in the bud dormancy–activity cycle, implying that certain differentially expressed miRNAs play crucial roles in apical bud burst, such as csn-miR319c/TCP2.

Abstract

microRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression by targeting mRNA transcripts for cleavage or directing translational inhibition. To investigate whether miRNAs regulate bud dormancy–activation transition in tea plant, which largely affects the yield and price of tea products and adaptability of tea trees, we constructed small RNA libraries from three different periods of bud dormancy–burst transition. Through sequencing analysis, 262 conserved and 83 novel miRNAs were identified, including 118 differentially expressed miRNAs. Quantitative RT-PCR results for randomly selected miRNAs exhibited that our comprehensive analysis is highly reliable and accurate. The content of caffeine increased continuously from the endodormancy bud to flushing bud, and differentially expressed miRNAs coupling with their targets associated with bud burst were identified. Remarkably, csn-miR319c was downregulated significantly from the quiescent bud to burst bud, while its target gene CsnTCP2 (TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR 2) displayed opposite expression patterns. Co-transformation experiment in tobacco demonstrated that csn-miR319c can significantly suppress the functions of CsnTCP2. This study on miRNAs and the recognition of target genes could provide new insights into the molecular mechanism of the bud dormancy–activation transition in tea plant.

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Abbreviations

ARF :

Auxin response factor

CTK:

Cytokinin

DAB/OAB/SAB:

Dormant/opened/swollen apical bud

ETH:

Ethylene

TCP:

TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31800585), the China Postdoctoral Science Foundation (2017M621991), the Anhui Provincial Natural Science Foundation (1808085QC92) and the Natural Science Foundation of Anhui Provincial Department of Education (KJ2018A0131). We appreciate the anonymous reviewers for constructive comments on this manuscript.

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  1. Shengrui Liu, Xiaozeng Mi and Ran Zhang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China

    Shengrui Liu, Xiaozeng Mi, Yanlin An, Tianyuan Yang, Xiaobo Xia, Rui Guo & Chaoling Wei

  2. Tea Research Institution, Anhui Academy of Agricultural Sciences, Huangshang, China

    Ran Zhang

  3. Henan Key Laboratory of Tea Plant Biology, Xinyang Normal University, 237 Nanhu Road, Xinyang, 464000, China

    Qiying Zhou

  4. Department of Genetics, University of Georgia, Athens, GA, 30602, USA

    Xuewen Wang

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  1. Shengrui Liu
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Liu, S., Mi, X., Zhang, R. et al. Integrated analysis of miRNAs and their targets reveals that miR319c/TCP2 regulates apical bud burst in tea plant (Camellia sinensis). Planta 250, 1111–1129 (2019). https://doi.org/10.1007/s00425-019-03207-1

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