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Expression of ethylene response genes during persimmon fruit astringency removal

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Abstract

Thirteen ethylene signaling related genes were isolated and studied during ripening of non-astringent ‘Yangfeng’ and astringent ‘Mopan’ persimmon fruit. Some of these genes were characterized as ethylene responsive. Treatments, including ethylene and CO2, had different effects on persimmon ripening, but overlapping roles in astringency removal, such as increasing the reduction in levels of soluble tannins. DkERS1, DkETR2, and DkERF8, may participate in persimmon fruit ripening and softening. The expression patterns of DkETR2, DkERF4, and DkERF5 had significant correlations with decreases in soluble tannins in ‘Mopan’ persimmon fruit, suggesting that these genes might be key components in persimmon fruit astringency removal and be the linkage between different treatments, while DkERF1 and DkERF6 may be specifically involved in CO2 induced astringency removal. The possible roles of ethylene signaling genes in persimmon fruit astringency removal are discussed.

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Abbreviations

ETR:

Ethylene receptor

CTR:

Constitutive triple response

EIL:

EIN3-like

ERF:

Ethylene responsive factor

PCNA:

Pollination-constant non-astringency

PCA:

Pollination-constant astringency

PDC:

Pyruvate decarboxylase

PVNA:

Pollination-variant non-astringency

PVA:

Pollination-variant astringency

ADH:

Alcohol dehydrogenase

1-MCP:

1-Methylcyclopropene

HRE:

Hypoxia responsive ERF

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Acknowledgments

We wish to thank Dr. J. Zhang (Beijing University of Agriculture, China) for support on collection of ‘Mopan’ fruit. This research was supported by the National Natural Science Foundation of China (no. 31030052, no. 31101507), the Program for Key Innovative Research Team of Zhejiang Province (no. 2009R50036), the Special Fund for Agro-scientific Research in the Public Interest (no. 201203047), the 111 project, and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People’s Republic of China

    Xue-ren Yin, Yan-na Shi, Ting Min, Qian Xu, Ian Ferguson & Kun-song Chen

  2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Zheng-rong Luo

  3. Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Yun-cong Yao

  4. New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland, New Zealand

    Ian Ferguson

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  1. Xue-ren Yin
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  2. Yan-na Shi
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Correspondence to Kun-song Chen.

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X. Yin and Y. Shi contributed equally to this work.

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Yin, Xr., Shi, Yn., Min, T. et al. Expression of ethylene response genes during persimmon fruit astringency removal. Planta 235, 895–906 (2012). https://doi.org/10.1007/s00425-011-1553-2

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