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Apple ethylene receptor protein concentrations are affected by ethylene, and differ in cultivars that have different storage life

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Abstract

Ethylene plays a crucial role in apple fruit ripening. Ethylene receptors have been identified and are known to be negative regulators of ethylene signalling. We examined ethylene receptors MdERS1 and MdERS2 in 1-MCP-treated and untreated fruit and leaves of cultivar ‘Orin’ and ‘Fuji’ apples. MdERS1 and MdERS2 transcription increased rapidly after harvest in control fruit, but in 1-MCP-treated fruit, increases were delayed for 30 days. However, MdERS1 and MdERS2 protein levels behaved differently. MdERS1 decreased gradually in both the control and 1-MCP treatments. MdERS2, however, increased gradually in control ‘Fuji’ and remained steady in 1-MCP-treated ‘Fuji’ but remained low in ‘Orin’. Exogenous ethylene treatment of fruit increased MdERS1 and MdERS2 expression with slightly decreased protein levels. The ratios of proteins to mRNAs were much lower in ‘Orin’ fruit, and they decreased with ethylene treatment in both cultivars. However, protein to transcript ratio was higher in ‘Fuji’ ethylene treated fruit than in air- and ethylene-treated ‘Orin’ fruit. MdERS1 and MdERS2 transcript levels were increased by exogenous ethylene treatment in air pre-treated leaves, but MdERS1 and MdERS2 protein levels did not change or decrease with ethylene treatment, and the ratio of protein to mRNA was lower in ethylene-treated leaves. Differences between transcription and protein levels may be due to receptor turnover differences in the presence or absence of ethylene. Furthermore, MdERS1 and MdERS2 protein stabilities in the presence of ethylene were different in the two cvs. ‘Orin’ and ‘Fuji’.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

ACO:

ACC oxidase

ACS:

ACC synthase

DAH:

Days after harvest

DTT:

Dithiothreitol

ER:

Endoplasmic reticulum

HRP:

Horseradish peroxidase

1-MCP:

1-Methylcyclopropene

PG:

Polygalacturonase

PVDF:

Polyvinylidene fluoride

RT-PCR:

Reverse transcription PCR

SDS:

Sodium dodecylsulphate

Trx:

Thioredoxin

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Acknowledgments

We thank Y. Kashimura (National Institute of Fruit Tree Science) for his help with 1-MCP treatment, and A. Endo (Fukushima Fruit Tree Experiment Station) for his gift of apple fruit. This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid for Young Scientists no. 15780031 to Miho Tatsuki).

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Authors and Affiliations

  1. National Institute of Fruit Tree Science, NARO, Fujimoto, 2-1, 305-8605, Tsukuba, Ibaraki, Japan

    Miho Tatsuki, Hiroko Hayama & Yuri Nakamura

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  1. Miho Tatsuki
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  2. Hiroko Hayama
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  3. Yuri Nakamura
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Correspondence to Miho Tatsuki.

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Tatsuki, M., Hayama, H. & Nakamura, Y. Apple ethylene receptor protein concentrations are affected by ethylene, and differ in cultivars that have different storage life. Planta 230, 407–417 (2009). https://doi.org/10.1007/s00425-009-0953-z

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  • DOI: https://doi.org/10.1007/s00425-009-0953-z

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