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Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains

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Abstract

To gain better wheat (Triticum aestivum L.) varieties for planting in Sichuan province, we compared the differences in the photosystem II (PSII) and antioxidant defense system at the blooming stage in 20 different new wheat strains collected from the wheat regional trial of Sichuan province in the year 2015. According to all measured data, we found that one of the strains, CD012J1, presented the highest photosynthetic and antioxidant enzyme activities, and a lower level of reactive oxygen species (ROS) than other wheat strains. In contrast, Chuan12147 had a lower photosynthetic rate and accumulated a higher level of ROS compared with other wheat strains. At the same time, we also found that wheat strains SH1103, 12C, XK322-1, and 13B4 had better photosynthetic capacity compared with Chuan12147. Correlation analysis indicated that wheat yield was significantly correlated with chlorophyll fluorescence parameters: the maximum efficiency of PSII photochemistry (F v/F m), the quantum yield of PSII electron transport, and the non-photochemical quenching coefficient. In addition, immunoblotting analysis indicated that Chuan12147 presented the highest levels of PsbS protein in six different wheat strains. Taken together, our results suggest that CD012J1 and Chuan12147 have the best and worst photosynthetic capacity and antioxidant systems, respectively. Moreover, F v/F m and PsbS protein could be used as a marker in breeding of wheat varieties.

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Acknowledgements

This research was supported by National Natural Science Foundation of China, (Projects Nos 31201149, 31300207 and 31171557). We are especially grateful to Dr Jian Li (Ohio University) for critical reading of the manuscript and the language editing of the manuscript.

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    1. College of Life Sciences, Sichuan Agricultural University, Ya’an, 625014, China

      Yang-Er Chen, Chao-Ming Zhang, Jie Ma, Hao-Tian Mao, Zi-Han Yang, Ming Yuan & Huai-Yu Zhang

    2. College of Life Science, Sichuan University, Chengdu, 610064, China

      Yan-Qiu Su

    3. College of Resources Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China

      Zhong-Wei Zhang & Shu Yuan

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    344_2017_9731_MOESM1_ESM.jpg

    Fig. S1 The maximum efficiency of PSII photochemistry (Fv/Fm) in 20 wheat plants at room temperature. Quantitative values (±SD) are shown below the individual fluorescence images (JPG 1418 KB)

    344_2017_9731_MOESM2_ESM.jpg

    Fig. S2 The quantum yield of PSII electron transport (ΦPSII) in 20 wheat plants at room temperature. Quantitative values (±SD) are shown below the individual fluorescence images (JPG 1302 KB)

    344_2017_9731_MOESM3_ESM.jpg

    Fig. S3 The non-photochemical quenching coefficient (NPQ) (C) in 20 wheat plants at room temperature. Quantitative values (±SD) are shown below the individual fluorescence images (JPG 1557 KB)

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    Chen, YE., Su, YQ., Zhang, CM. et al. Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul 37, 347–359 (2018). https://doi.org/10.1007/s00344-017-9731-5

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