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Fine mapping of the qLOP2 and qPSR2-1 loci associated with chilling stress tolerance of wild rice seedlings

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Abstract

Key message

Using leaf osmotic potential and plant survival rate as chilling-tolerant trait indices, we identified two major quantitative trait loci qLOP2 and qPSR2 - 1 (39.3-kb region) and Os02g0677300 as the cold-inducible gene for these loci.

Abstract

Chilling stress tolerance (CST) at the seedling stage is an important trait affecting rice production in temperate climate and high-altitude areas. To identify quantitative trait loci (QTLs) associated with CST, a mapping population consisting of 151 BC2F1 plants was constructed by using chilling-tolerant Dongxiang wild rice (Oryza rufipogon Griff.) as a donor parent and chilling-sensitive indica as a recurrent parent. With leaf osmotic potential (LOP) and plant survival rate (PSR) as chilling-tolerant trait indexes, two major QTLs, qLOP2 (LOD = 3.8) and qPSR2-1 (LOD = 3.3), were detected on the long arm of chromosome 2 by composite interval mapping method in QTL Cartographer software, which explained 10.1 and 12.3 % of the phenotypic variation, respectively. In R/QTL analyzed result, their major effects were also confirmed. Using molecular marker RM318 and RM106, qLOP2 and qPSR2-1 have been introgressed into chilling-sensitive varieties (93-11 and Yuefeng) by marker-assisted selection procedure (MAS), which resulted in 16 BC5F3 BILs that chilling tolerance have significantly enhanced compare with wild-type parents (P < 0.01). Therefore, two large segregating populations of 11,326 BC4F2 and 8,642 BC4F3 were developed to fine mapping of qLOP2 and qPSR2-1. Lastly, they were dissected to a 39.3-kb candidate region between marker RM221 and RS8. Expression and sequence analysis results indicated that Os02g0677300 was a cold-inducible gene for these loci. Our study provides novel alleles for improving rice CST by MAS and contributes to the understanding of its molecular mechanisms.

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Abbreviations

CST:

Chilling stress tolerance

QTL:

Quantitative trait locus

PSR:

Plant survival rate

LOP:

Leaf osmotic potential

BIL:

Backcross introgression lines

CBF:

Calmodulin-binding transcription activator

DREB:

Dehydration-responsive element binding

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Acknowledgments

This study was supported by National Natural Science Foundation of China (31401365), Natural Science Foundation (BK2011426 and BK2011427) of Jiangsu Province, National Modern Agricultural Industry Technology System Special Fund (CARS-01-45), and the Priority Academic Program Development of Jiangsu Higher Education Institutions and International Atomic Energy Agency (12228/RO).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology and Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, Jiangsu Province, China

    Ning Xiao, Wei-nan Huang, Yong Gao, Yi Dai & Jian-min Chen

  2. Lixiahe Agricultural Research Institute of Jiangsu Province, National Rice Industry Technology System of Yangzhou Comprehensive Experimental Station, Yangzhou, Jiangsu Province, China

    Ning Xiao, Ai-hong Li, Yu-hong Li, Cun-hong Pan, Hongjuan Ji, Xiao-xiang Zhang & Zheng-yuan Dai

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  1. Ning Xiao
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  2. Wei-nan Huang
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  11. Jian-min Chen
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Corresponding authors

Correspondence to Zheng-yuan Dai or Jian-min Chen.

Additional information

Communicated by Matthias Wissuwa.

N. Xiao and W. Huang have contributed equally to this work.

Electronic supplementary material

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Supplementary material 1 (DOC 154 kb)

Supplementary Fig. 1 The sequence blast of coding region between Dongxiang and Nanjing 11 (PDF 199 kb)

Supplementary Fig. 2 The protein sequences blast between Dongxiang and Nanjing 11 (PDF 143 kb)

122_2014_2420_MOESM4_ESM.tif

Supplementary Fig. 3 Expression analysis of OsLti6a, OsLti6b, and P5CS by real-time PCR for plants maintained at 4°C for different durations. *Significant difference from Nanjing 11 with a P value = 0.01 (TIFF 4690 kb)

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Xiao, N., Huang, Wn., Li, Ah. et al. Fine mapping of the qLOP2 and qPSR2-1 loci associated with chilling stress tolerance of wild rice seedlings. Theor Appl Genet 128, 173–185 (2015). https://doi.org/10.1007/s00122-014-2420-x

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