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Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress

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Abstract

To understand the functions of transcription factor OsNAC5 in response to abiotic stress, we generated transgenic rice plants with knockdown OsNAC5 by RNA-interfered (RNAi) and overexpressing OsNAC5, and investigated the effects of cold, drought and salt stress on wild-type (WT), RNAi and overexpression rice lines. Our results demonstrated that RNAi lines became less tolerant to these stresses than WT plants, while overexpression of OsNAC5 in Arabidopsis and rice enhanced tolerance to these stresses. The mechanisms underlying the changes in tolerance of the transgenic rice plants to abiotic stresses were explored by measuring free proline (Pro) and soluble sugar contents in WT and transgenic plants. Accumulation of Pro and soluble sugars was positively correlated with OsNAC5 expression levels. The less accumulation of Pro in RNAi lines may be accounted for by inhibition of Pro synthesis and transport at transcriptional levels. In addition, knockdown and overexpression of OsNAC5 enhanced and reduced accumulation of malondialdehyde and H2O2, suggesting that knockdown of OsNAC5 renders RNAi plants more susceptible to oxidative damage. The RNAi lines displayed higher Na+/K+ ratio due to greater accumulation of Na+ ions than WT under salt stress conditions, and expression of genes encoding tonoplast Na+/H+ antiporter was lower in RNAi lines than in WT under both control and salt-stressed conditions. Seed germination of RNAi and overexpression plants was more and less inhibited by salt and mannitol than that of WT, respectively. Seed germination of overexpression and RNAi plants was more and less sensitive than that of WT to ABA. These findings highlight the important role of OsNAC5 played in the tolerance of rice plants to abiotic stress by regulating downstream targets associated with accumulation of compatible solutes, Na+ ions, H2O2 and malondialdehyde.

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Abbreviations

ABA:

Abscisic acid

CUC:

Cup-shaped cotyledon

GUS:

β-Glucuronidase

MDA:

Malondialdehyde

MeJA:

Methyl jasmonic acid

MS:

Murashige and Skoog

NAC:

NAM, ATAF and CUC

NAM:

No apical meristem

NHX:

Na+/H+ exchanger

P5CS:

Δ1-Pyrroline-5-carboxylate synthetase

PEG:

Polyethylene glycol

Pro:

Proline

SNAC:

Stress-responsive NAC

TPP:

Trehalose-6-phosphate phosphatase

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Acknowledgments

This work was supported by the National Science Foundation of China (30788003&30870188) and the Chinese Academy of Science (Grant No. KSCX1-YW-03).

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Authors and Affiliations

  1. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People’s Republic of China

    Shi-Yong Song, Ying Chen, Jie Chen, Xiao-Yan Dai & Wen-Hao Zhang

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  1. Shi-Yong Song
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  2. Ying Chen
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  3. Jie Chen
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Correspondence to Wen-Hao Zhang.

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The authors S.-Y. Song and Y. Chen contributed equally to this work.

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Song, SY., Chen, Y., Chen, J. et al. Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress. Planta 234, 331–345 (2011). https://doi.org/10.1007/s00425-011-1403-2

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