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Characterization of the quantitative trait locus OilA1 for oil content in Brassica napus

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Abstract

Increasing seed oil content has become one of the most important breeding criteria in rapeseed (Brassica napus). However, oil content is a complex quantitative trait. QTL mapping in a double haploid population (SG population) emerging from a cross between a German (Sollux) and Chinese (Gaoyou) cultivars revealed one QTL for oil content on linkage group A1 (OilA1), which was mapped to a 17 cM genetic interval. To further validate and characterize the OilA1, we constructed a high-resolution map using B. rapa sequence resources and developed a set of near-isogenic lines (NILs) by employing a DH line SG-DH267 as donor and Chinese parent Gaoyou as recurrent background. The results showed highly conserved synteny order between B. rapa and B. napus within the linkage group A1 and revealed a possible centromere region between two markers ZAASA1-38 and NTP3 (2.5 cM). OilA1 was firstly validated by 250 BC5F2 plants and was confirmed in a 10.6 cM interval between the markers ZAASA1-47 and ZAASA1-77. Further substitution mapping was conducted by using two generations of QTL-NILs, 283 lines from eight BC5F3:4 families and 428 plants from six BC5F4 sub-NILs and thus narrowed the OilA1 interval to 6.9 cM and 4.3 cM (1.4 Mb), respectively. Field investigations with two replications using homozygous BC5F3:4 sister sub-NILs indicated that NILs, which carry a Sollux chromosome segment across the target region showed significant higher oil content (1.26 %, p < 0.001) than their sister NILs containing Gaoyou chromosome. The OilA1 locus is of particular interest for breeding purpose in China because 80 % of Chinese cultivars do not carry this desirable allele.

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Acknowledgments

The authors are grateful to Dr. Christian Moellers (Georg-August-Universität Göttingen), Dr Amine Abbadi (Norddeutsche Pflanzenzucht Hans-Georg Lembke KG) and Dr. Yunhai Li (Chinese Academy of Sciences) for their critical reading of the manuscript. This research was financially supported by National Natural Science Foundation of China (No. 31171180), Zhejiang Provincial Natural Science Foundation of China (Z3100592) National High-tech R&D Program (2011AA10A104) and the project funded by Science Technology Department of Zhejiang Province (2011C12005, 2012C12902).

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Authors and Affiliations

  1. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

    Yubo Chen, Lu Qi, Xiaoyu Zhang, Jixiang Huang, Jibian Wang, Hongcheng Chen, Xiyuan Ni & Jianyi Zhao

  2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

    Yubo Chen, Lu Qi & Xiaoyu Zhang

  3. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China

    Jibian Wang & Haiming Xu

  4. School of Life Sciences, Anhui Agricultural University, Hefei, China

    Hongcheng Chen

  5. Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

    Fei Xu

  6. College of Life and Environment Sciences, Shanghai Normal University, Shanghai, China

    Lu Qi & Yanjun Dong

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  1. Yubo Chen
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  10. Haiming Xu
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Correspondence to Jianyi Zhao.

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Communicated by C. Quiros.

Y. B. Chen and L. Qi contributed equally to this work.

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Chen, Y., Qi, L., Zhang, X. et al. Characterization of the quantitative trait locus OilA1 for oil content in Brassica napus . Theor Appl Genet 126, 2499–2509 (2013). https://doi.org/10.1007/s00122-013-2150-5

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