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Fine mapping of a grain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross between Oryza sativa and Oryza rufipogon

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Abstract

A quantitative trait locus (QTL) for grain weight (GW) was detected near SSR marker RM210 on chromosome 8 in backcross populations derived from a cross between the Korean japonica cultivar Hwaseongbyeo and Oryza rufipogon (IRGC 105491). The O. rufipogon allele increased GW in the Hwaseongbyeo background despite the fact that O. rufipogon was the small-seeded parent. Using sister BC3F3 near-isogenic lines (NILs), gw8.1 was validated and mapped to a 6.1 cM region in the interval between RM42 and RM210 (P≤0.0001). Substitution mapping with eight BC3F4 sub-NILs further narrowed the interval containing gw8.1 to about 306.4 kb between markers RM23201.CNR151 and RM30000.CNR99. A yield trial using homozygous BC3F4 sister sub-NILs and the Hwaseongbyeo recurrent parent indicated that the NIL carrying an O. rufipogon chromosome segment across the entire gw8.1 target region out-yielded its sister NIL (containing Hwaseongbyeo chromosome in the RM42–RM210 interval) by 9% (P=0.029). The higher-yielding NIL produced 19.3% more grain than the Hwaseongbyeo recurrent parent (P=0.018). Analysis of a BC3F4 NIL indicated that the variation for GW is associated with variation in grain shape, specifically grain length. The locus, gw8.1 is of particular interest because of its independence from undesirable height and grain quality traits. SSR markers tightly linked to the GW QTL will facilitate cloning of the gene underlying this QTL as well as marker-assisted selection for variation in GW in an applied breeding program.

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Acknowledgments

This study was supported by grants from the Bio Green 21 project of the Rural Development Administration and from the Crop Functional Genomics Center of the 21st Century Frontier Research Program (Project code no. CG3112) funded by the Ministry of Science and Technology, Republic of Korea.

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  1. Xiaobo Xie

    Present address: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea

    Xiaobo Xie, Mi-Hee Song, Fengxue Jin & Sang-Nag Ahn

  2. National Institute of Crop Science, Rural Development Administration, Suweon, 441-100, Korea

    Jung-Pil Suh & Hung-Goo Hwang

  3. Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, 14850, USA

    S. R. McCouch

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  1. Xiaobo Xie
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Correspondence to Sang-Nag Ahn.

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Communicated by Y. Xue

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Xie, X., Song, MH., Jin, F. et al. Fine mapping of a grain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross between Oryza sativa and Oryza rufipogon . Theor Appl Genet 113, 885–894 (2006). https://doi.org/10.1007/s00122-006-0348-5

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