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Identification of QTL for oil content, seed yield, and flowering time in oilseed rape (Brassica napus)

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Abstract

A genetic map was constructed with 353 sequence-related amplified polymorphism and 34 simple sequence repeat markers in oilseed rape (Brassica napus L.). The map consists of 19 linkage groups and covers 1,868 cM of the rapeseed genome. A recombinant doubled haploid (DH) population consisting of 150 lines segregating for oil content and other agronomic traits was produced using standard microspore culture techniques. The DH lines were phenotyped for days to flowering, oil content in the seed, and seed yield at three locations for 3 years, generating nine environments. Data from each of the environments were analyzed separately to detect quantitative trait loci (QTL) for these three phenotypic traits. For oil content, 27 QTL were identified on 14 linkage groups; individual QTL for oil content explained 4.20–30.20% of the total phenotypic variance. For seed yield, 18 QTL on 11 linkage groups were identified, and the phenotypic variance for seed yield, as explained by a single locus, ranged from 4.61 to 24.44%. Twenty-two QTL were also detected for days to flowering, and individual loci explained 4.41–48.28% of the total phenotypic variance.

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Abbreviations

DH:

Doubled haploid

QTL:

Quantitative trait loci

CIM:

Composite interval mapping

SRAP:

Sequence-related amplified polymorphism

SSR:

Simple sequence repeat

LG:

Linkage group

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Acknowledgements

This work was supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada and industrial and government partners, through the Green Crop Networks (GCN) Research Network Canada.

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Authors and Affiliations

  1. Department of Plant Science, University of Manitoba, 222 Agriculture Building, 66 Dafoe Road, Winnipeg, MB, Canada

    Gang Chen, Jianfeng Geng, Xueping Liu, Gengyi Li, Peter B. E. McVetty & M. Tahir

  2. Huazhong Agricultural University, Wuhan, China

    Jingxing Tu & Tingdong Fu

  3. Department of Plant Science, North Dakota State University, 370B Loftsgard Hall, Fargo, ND, 58105-5051, USA

    Mukhlesur Rahman

Authors
  1. Gang Chen
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  2. Jianfeng Geng
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  3. Mukhlesur Rahman
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  4. Xueping Liu
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  5. Jingxing Tu
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  6. Tingdong Fu
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  7. Gengyi Li
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  8. Peter B. E. McVetty
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  9. M. Tahir
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Correspondence to M. Tahir.

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Chen, G., Geng, J., Rahman, M. et al. Identification of QTL for oil content, seed yield, and flowering time in oilseed rape (Brassica napus). Euphytica 175, 161–174 (2010). https://doi.org/10.1007/s10681-010-0144-9

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  • DOI: https://doi.org/10.1007/s10681-010-0144-9

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