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Identification and mapping of a major dominant quantitative trait locus controlling seeds per silique as a single Mendelian factor in Brassica napus L.

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Abstract

One putative quantitative trait locus (QTL) for seeds per silique (SS), cqSS.A8, was identified using a double haploid (DH) population in Brassica napus, and near-isogenic lines (NILs; BC3F1) for cqSS.A8 were developed. However, the flanking markers from cqSS.A8 showed no significant difference using single-marker analysis, even though the frequency distribution of SS in the BC3F1 was bimodal, suggesting that one novel locus existed. In this study, we characterized the effects of this locus in the NILs and used a published linkage map to determine its location. A three-step approach was designed for mapping the locus in the NILs (BC3F2): (1) determining the individual BC3F2 genotype at the locus using a progeny test; (2) identifying amplified fragment length polymorphism (AFLP) markers linked to the locus using a combination of AFLP and bulked segregant analysis; and (3) determining the location and effects of this locus. QTL analysis in the BC3F2 revealed that this locus explained 85.8 and 55.7 % of phenotypic variance for SS and SL, respectively. Its additive and dominant effects on SS were 6.1 and 5.7, respectively. The locus was validated using a DH population by composite interval mapping and located to linkage group C9 (designated as qSS.C9). Mapping qSS.C9 was undertaken using 230 extremely low-SS plants of a BC4F1 population containing 807 plants. We found that qSS.C9 delimited a 1.005-Mb interval including 218 predicted genes in the reference Brassica rapa (Chiifu-401). These results will greatly facilitate map-based cloning of qSS.C9 and seed yield improvement in rapeseed.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

CB-NILs:

Consecutive backcrossing near-isogenic lines

DH:

Double haploid

HIF-NILs:

Heterogeneous inbred family near-isogenic lines

LOD:

Logarithm of odds score

NIL:

Near-isogenic line

QTL:

Quantitative trait locus

SCAR:

Sequence characterized amplified region

SSR:

Simple sequence repeats

SS :

Seeds per silique

SL :

Silique length

SW :

1,000-seed weight

TP-MBS:

Trait performance and marker-assisted background selection

TP-NILs:

Trait performance near-isogenic lines

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Acknowledgments

This project was sponsored by funds from China Postdoctoral Science Foundation (No. 2010048091), the National Natural Science Foundation of China (No. 31100883), and the National High Technology Research and Development Program of China (863 Program; No. 2011AA10A104).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, Hubei, People’s Republic of China

    Liwu Zhang, Shipeng Li, Lei Chen & Guangsheng Yang

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  1. Liwu Zhang
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  2. Shipeng Li
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  3. Lei Chen
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  4. Guangsheng Yang
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Correspondence to Guangsheng Yang.

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Communicated by C. Quiros.

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Zhang, L., Li, S., Chen, L. et al. Identification and mapping of a major dominant quantitative trait locus controlling seeds per silique as a single Mendelian factor in Brassica napus L.. Theor Appl Genet 125, 695–705 (2012). https://doi.org/10.1007/s00122-012-1861-3

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  • DOI: https://doi.org/10.1007/s00122-012-1861-3

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