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A framework genetic map of Muscadinia rotundifolia

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Abstract

This study presents a framework linkage map based on microsatellite markers for Muscadinia rotundifolia (1n = 20). The mapping population consisted of 206 progeny generated from a cross of two M. rotundifolia varieties, ‘Fry’ and ‘Trayshed’. A total of 884 primers were tested for their ability to amplify markers: 686 amplified and 312 simple sequence repeat (SSR) primer pairs generated 322 polymorphic markers for either one or both parents. The map for the female parent ‘Fry’ consisted of 212 markers and covered 879 cM on 18 chromosomes. The average distance between the markers was 4.1 cM and chromosome 6 was not represented due to a lack of polymorphic markers. The map for the male parent ‘Trayshed’ consisted of 191 markers and covered 841 cM on 19 chromosomes. The consensus map consisted of 314 markers on 19 chromosomes with a total distance of 1,088 cM, which represented 66 % of the distance covered by the Vitis vinifera reference linkage map. Marker density varied greatly among chromosomes from 5 to 35 mapped markers. Relatively good synteny was observed across 19 chromosomes based on markers in common with the V. vinifera reference map. Extreme segregation distortion was observed for chromosome 8 and 14 on the female parent map, and 4 on the male parent map. The lack of mapping coverage for the 20th M. rotundifolia chromosome is discussed in relation to possible evolutionary events that led to the reduction in chromosome number from 21 to 19 in the ancestral genome.

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Acknowledgments

Research funding from the California Grape Rootstock Improvement Commission, the California Grapevine Rootstock Research Foundation, the California Department of Food and Agriculture PD/GWSS Board and the Louis P. Martini Endowed Chair funds is gratefully acknowledged. The authors also thank Dr. John E. Bowers, Institute of Plant Breeding Genetics and Genomics, University of Georgia, and Didier Merdinoglu, Institut National de la Recherche Agronomique—Colmar, for helpful suggestions on the manuscript.

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  1. Department of Viticulture and Enology, University of California, Davis, CA, 95616, USA

    S. Riaz, R. Hu & M. A. Walker

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Correspondence to M. A. Walker.

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Communicated by A. Charcosset.

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Supplementary Figure 1. Pedigree of the M. rotundifolia cultivar Fry (PPT 80 kb)

122_2012_1906_MOESM2_ESM.ppt

Supplementary Figure 2. Comparison of the consensus genetic map of M. rotundifolia (center) to two different maps of V. vinifera. On the left (A) is the integrated map (Doligez et al. 2006) of five different grape populations: 1) Syrah × Grenache; 2) Selfed Riesling; 3) Chardonnay × Bianca (Bouvier × Villard Blanc); 4) MTP2223-27 (Dattier de Beyrouth × 75 Pirovano) × MTP2121-30 (Alphonse Lavallée × Sultanine); and 5) Riesling × Cabernet Sauvignon. The map on the right (B) is a consensus of two populations (Chardonnay × Bianca (Bouvier × Villard Blanc), and Cabernet Sauvignon × Vitis ‘breeding line 20/3’. Lines join markers common among the three maps. Marker names in red indicate those SSR primer pairs that amplified V. vinifera ‘Thompson Seedless’ but failed to generate any product for the Muscadinia samples (PPT 4242 kb)

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Riaz, S., Hu, R. & Walker, M.A. A framework genetic map of Muscadinia rotundifolia . Theor Appl Genet 125, 1195–1210 (2012). https://doi.org/10.1007/s00122-012-1906-7

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