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Isolation of (AC)n-microsatellites in Vitis vinifera L. and analysis of genetic background in grapevines under marker assisted selection

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Abstract

Primers were developed for 118 microsatellites isolated from grape (Vitis vinifera) genomic libraries enriched for (AC)n repeats. Only one microsatellite sequence matched other grape SSR-sequences in the GeneBank database. Genotyping was carried out in the parental lines and four offspring of two pseudo-test-cross populations, ‘Cabernet Sauvignon’ x ‘Seyval’ and ‘Chardonnay’ x ‘Bianca’, and a further six other grape genotypes (V. vinifera ‘Sultanina’, ‘Merlot’, ‘Syrah’, ‘Müller-Thurgau’, Vitis ‘Regent’ and V. riparia ‘Gloire de Montpellier’). A total of 108 microsatellites showed easily scorable alleles and 100 of them segregated according to a configuration suitable for mapping in either cross. A further 8 SSRs, although unsuitable for mapping in those crosses, showed polymorphism in the other genotypes tested. This set of markers was used, along with 75 microsatellites of other repeat-types, to fingerprint 46 offspring of the cross ‘Chardonnay’ x ‘Bianca’. For each full-sib, individual heterozygosity and distance in repeat units between pairs of alleles at each locus (mean d2) were calculated as a tool for predicting ‘highly outbred’ recombinant individuals. Six microsatellites with segregation ratios significantly distorted towards the lack of homozygous sibs were identified and mapped to linkage groups LG 3 and LG 5. Estimation of heterozygosity at genome-wide level and genotyping at loci for which homozygous sibs are discriminated against are discussed for marker-assisted background selection in outcrossing grapevines.

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie e Ambientali, University of Udine, Via delle Scienze 208, 33100, Udine, Italy

    Gabriele Di Gaspero, Guido Cipriani, Maria Teresa Marrazzo, Daniele Andreetta, Maria Jesus Prado Castro, Enrico Peterlunger & Raffaele Testolin

  2. INIA-CIFOR, Crta., de la Coruña, 28040, Madrid, Spain

    Maria Jesus Prado Castro

Authors
  1. Gabriele Di Gaspero
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  2. Guido Cipriani
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  3. Maria Teresa Marrazzo
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  4. Daniele Andreetta
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  5. Maria Jesus Prado Castro
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  6. Enrico Peterlunger
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  7. Raffaele Testolin
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Correspondence to Gabriele Di Gaspero.

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Di Gaspero, G., Cipriani, G., Marrazzo, M.T. et al. Isolation of (AC)n-microsatellites in Vitis vinifera L. and analysis of genetic background in grapevines under marker assisted selection. Mol Breeding 15, 11–20 (2005). https://doi.org/10.1007/s11032-004-1362-4

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  • DOI: https://doi.org/10.1007/s11032-004-1362-4

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