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A framework map from grapevine V3125 (Vitis vinifera ‘Schiava grossa’ × ‘Riesling’) × rootstock cultivar ‘Börner’ (Vitis riparia × Vitis cinerea) to localize genetic determinants of phylloxera root resistance

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Abstract

Grapevine rootstock cultivar ‘Börner’ is a hybrid of Vitis riparia and Vitis cinerea Arnold that shows high resistance to phylloxera (Daktulosphaira vitifoliae Fitch). To localize the determinants of phylloxera root resistance, the susceptible grapevine V3125 (Vitis vinifera ‘Schiava grossa’ × ‘Riesling’) was crossed to ‘Börner’. Genetic framework maps were built from the progeny. 235 microsatellite markers were placed on the integrated parental map. They cover 1,155.98 cM on 19 linkage groups with an average marker distance of 4.8 cM. Phylloxera resistance was scored by counting nodosities after inoculation of the root system. Progeny plants were triplicated and experimentally infected in 2 years. A scan of the genetic maps indicated a quantitative trait locus on linkage group 13. This region was targeted by six microsatellite-type markers newly developed from the V. vinifera model genome sequence. Two of these appear closely linked to the trait, and can be useful for marker-assisted breeding.

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Acknowledgments

The expert technical assistance of Andreas Preiss, Dagmar Andrä and Margrit Daum is highly acknowledged. Junke Zhang was funded as a visiting scientist under the frame of a Sino-Germany Cooperation by the Federal Ministry of Food, Agriculture and Consumer Protection and the Foundation of Friends and Promoters of the Institute for Grapevine Breeding Geilweilerhof.

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Author notes

  1. Junke Zhang

    Present address: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

  2. Leocir J. Welter

    Present address: Centro de Ciencias Agrarias (CCA) Rodovia Admar Gonzaga, Universidade Federal de Santa Catarina (UFSC), 1346, Itacorubi, Florianópolis, SC, 88034-001, Brazil

Authors and Affiliations

  1. Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, Germany

    Junke Zhang, Ludger Hausmann, Rudolf Eibach, Leocir J. Welter, Reinhard Töpfer & Eva M. Zyprian

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  1. Junke Zhang
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  2. Ludger Hausmann
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  3. Rudolf Eibach
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  4. Leocir J. Welter
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Correspondence to Eva M. Zyprian.

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Communicated by A. Graner.

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122_2009_1107_MOESM1_ESM.xls

Primer list of the STMS markers specifically developed for linkage group 13 based on the genome sequence of PN40024 (sc = scaffold) (XLS 19 kb)

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Zhang, J., Hausmann, L., Eibach, R. et al. A framework map from grapevine V3125 (Vitis vinifera ‘Schiava grossa’ × ‘Riesling’) × rootstock cultivar ‘Börner’ (Vitis riparia × Vitis cinerea) to localize genetic determinants of phylloxera root resistance. Theor Appl Genet 119, 1039–1051 (2009). https://doi.org/10.1007/s00122-009-1107-1

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  • DOI: https://doi.org/10.1007/s00122-009-1107-1

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