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Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew

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Abstract

Downy mildew, caused by the oomycete Plasmopara viticola, is one of the major threats to grapevine. All traditional cultivars of grapevine (Vitis vinifera) are susceptible to downy mildew, the control of which requires regular application of fungicides. In contrast, many sources of resistance to P. viticola have been described in the Vitis wild species, among which is V. amurensis Rupr. (Vitaceae), a species originating from East Asia. A genetic linkage map of V. amurensis, based on 122 simple sequence repeat and 6 resistance gene analogue markers, was established using S1 progeny. This map covers 975 cM on 19 linkage groups, which represent 82% of the physical coverage of the V. vinifera reference genetic map. To measure the general level of resistance, the sporulation of P. viticola and the necrosis produced in response to infection, five quantitative and semi-quantitative parameters were scored 6 days post-inoculation on the S1 progeny. A quantitative trait locus (QTL) analysis allowed us to identify on linkage group 14 a major QTL controlling the resistance to downy mildew found in V. amurensis, which explained up to 86.3% of the total phenotypic variance. This QTL was named ‘Resistance to Plasmopara viticola 8’ (Rpv8).

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Acknowledgments

This work was partially financed by the Agence Nationale de Recherche (ERA-NET Plant Genomics Program-GRASP GRAPE WINE 074B). We are grateful to E. Duchêne for useful discussions on statistical methods. We thank P. Coste, M.-A. Dorne and C. Onimus for excellent technical assistance in plant growing and inoculum maintenance.

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Authors and Affiliations

  1. UMR 1131 Santé de la Vigne et Qualité du Vin, Institut National de la Recherche Agronomique, 28 rue de Herrlisheim, BP 20507, 68021, Colmar Cedex, France

    Paule Blasi, Sophie Blanc, Sabine Wiedemann-Merdinoglu, Emilce Prado, Pere Mestre & Didier Merdinoglu

  2. UMR 1131 Santé de la Vigne et Qualité du Vin, Université de Strasbourg, 28 rue de Herrlisheim, BP 20507, 68021, Colmar, France

    Paule Blasi, Sophie Blanc, Sabine Wiedemann-Merdinoglu, Emilce Prado, Pere Mestre & Didier Merdinoglu

  3. Hochschule RheinMain, Geisenheim Research Center, von-Lade-Str. 1, 65366, Geisenheim, Germany

    Ernst H. Rühl

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  1. Paule Blasi
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  2. Sophie Blanc
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Correspondence to Didier Merdinoglu.

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Communicated by C. Gebhardt.

P. Blasi and S. Blanc contributed equally to the present work and should therefore be considered first co-authors.

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Blasi, P., Blanc, S., Wiedemann-Merdinoglu, S. et al. Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew. Theor Appl Genet 123, 43–53 (2011). https://doi.org/10.1007/s00122-011-1565-0

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