Abstract
The nuclear DNA content was analyzed in Vitis species, hybrid cultivars, and genera of the Vitaceae using flow cytometry. Significant variation was found among Vitis species, hybrids, and other genera of the Vitaceae (Ampelopsis and Parthenocissus). DNA content was estimated to range from 0.98 to 1.05 pg/2C within V. labrusca (ns) and 0.86 to 1.00 pg/2C within V. vinifera (ns). Genotypes from Vitis and Parthenocissus were similar in nuclear DNA content (approximately 1.00 pg/2C) whereas they differed significantly from Ampelopsis (1.39 pg/2C). No correlation between DNA content and the center of origin of genotypes of the Vitaceae was noted. Based on the present study, the Vitis genome size is 475 Mbp, 96% of which is non-coding. Knowledge of DNA content is useful in order to understand the complexity of the Vitis genome and to establish a relationship between the genetic and physical map for map-based cloning.
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References
Alleweldt G, Possingham JV (1988) Progress in grapeving breeding. Theor Appl Genet 75:669–673
Anonymous (1993) FAO Production Year Book, vol 46. Food and Agricultural Organization of the United Nations, Rome
Arumuganathan K, Earle ED (1991a) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–219
Arumuganathan K, Earle ED (1991b) Estimation of nuclear DNA content by flow cytometry. Plant Mol Biol Rep 9:229–241
Bachmann K, Price HJ (1977) Repetitive DNA in Cichorieae (Compositae). Chromosoma 61:267–275
Bennett MD (1972) Nuclear DNA content and minimum generation time in herbaceous plants. Proc R Soc Lond B181:109–135
Bennett MD (1985) Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In: Freeling M (ed) Plant genetics. A. R. Liss, New York, pp 283–302
Bennett MD (1987) Variation in genomic form in plants and its ecological implications. New Phytol 106:177–200
Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc Lond B274:227–274
Bennett MD, Gustafson JP, Smith JB (1977) Variation in nuclear DNA in the genus Secale. Chromosoma 61:149–176
Bennett MD, Smith JB, Ward JP, Finch RA (1982) The relationship between chromosome volume and DNA content in unsquashed metaphase cells of barley, Hordeum vulgare cv Tullen 346. J Cell Sci 56:101–111
Bhaskaran S, Swaminathan MS (1960) Metaphase chromosome length and DNA content in relation to polyploidy in Triticum species. Exp Cell Res 20:598–599
Cavallini A, Natali L, Cionini G, Gennai D (1993) Nuclear DNA variability within Pisum sativum (Leguminosae): nucleotypic effects on plant growth. Heredity 70:561–565
Ceccarelli M, Falistocco E, Cionini PG (1992) Variation in genome size and organization within hexaploid Festuca arundinaceae. Theor Appl Genet 83:271–278
Costich DE, Ortiz R, Meagher TR, Bruederle LP, Vorsa N (1993) Determination of ploidy level and nuclear DNA content in blue-berry by flow cytometry. Theor Appl Genet 86:1001–1006
Cullis CA (1990) DNA rearrangements in response to environmental stress. Adv Genet 28:73–97
Dickson EE, Arumuganathan K, Kresovich S, Doyle JJ (1992) Nuclear DNA content variation within the Rosaceae. Am J Bot 79:1081–1086
Figueira A, Janick J, Goldsbrough P (1992) Genome size and DNA polymorphism in Theobroma cacao. J Am Soc Hort Sci 117:673–677
Flavell RB (1980) The molecular characterization and organization of plant chromosomal DNA sequences. Annu Rev Pl Physiol 31:569–596
Flavell RB (1985) Repeated sequences and genome change. In: Hohn B, Dennis ES (eds) Genetic flux in plants. Springer-Verlag, Berlin Heidelberg, New York, pp 139–156
Flavell RB (1986) Repetitive DNA and chromosome evolution in plants. Phil Trans R Soc Lond B312:227–242
Flavell RB, Dean C, Moore G (1993a) Exploiting plant genomes, small and large. In: Heslop-Harrison JS, Flavell RB (eds) The chromosome. BIOS Scientific Publishers, UK, pp 249–264
Flavell RB, Gale MD, O'Dell M, Lucas H, Murphy G, Moore G (1993b) Molecular organization of genes and repeats in the large cereal genomes and implications for isolation of genes by chromosome walking. In: Chandley A, Sumner A (eds) Chromosomes today, Vol 11. Chapman and Hall, London, pp 199–213
Furuta Y, Nishikawa K (1991) Variation in nuclear and individual chromosomal DNA content and its role in evolution of plants. In: Gupta PK, Tsuchiya T (eds) Chromosome engineering in plants: genetics, breeding and evolution, Part A. Elsevier, New York, pp 71–86
Furuta Y, Nishikawa K, Makino T, Sawai Y (1984) Variation in DNA content of 21 individual chromosomes among six subspecies in common wheat. Jpn J Genet 59:83–90
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of cell cycle in intact plant tissues. Science 220:1049–1051
Galet P (1988) Cépages et vignobles de France, Tome 1. Les vignes Américaines, 2nd edn. Imprimerie Charles Déhan, Montpellier, France
Grime JP (1990) Ecological effects of climate change on plant populations and vegetation composition with particular reference to the British flora. In: Jackson M, Ford-Lloyd BV, Parry ML (eds) Climatic changes and plant genetic resources. Belhaven Press, London, pp 40–60
Grime JP, Mowforth MA (1982) Variation in genome size-an ecological interpretation. Nature 299:151–153
Grueilhuber J, Septa F (1985) Geographical variation of genome size at low taxonomic levels in Scilla bifolia alliance (Hyacinthaceae). Flora 176:431–438
Hain R, Reif HJ, Krause E, Langbartels R, Kindle H, Vornam B, Wiese W, Schmelzer E, Schreier PH, Stöcker RH, Stenzel K (1993) Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361:153–156
Hammatt N, Blackhall NW, Davey MR (1991) Variation in the DNA content of Glycine spp. J Exp Bot 42:659–666
Hébert D, Kikkert JR, Smith FD, Reisch BI (1993) Optimization of biolistic transformation of embryogenie grape-cell suspensions. Plant Cell Rep 12:585–589
Hutchinson J, Rees H, Seal AG (1979) An assay of the activity of supplementary DNA in Lolium. Heredity 43:507–509
Jones RN, Brown LM (1976) Chromosomal evolution and DNA variation in Crepis. Heredity 36:613–641
Kenton A (1984) Chromosomal evolution in the Gibasis linearis group (Commelinaceae). III. DNA variation, chromosomal evolution and speciation in G. venustula and G. heterophylla. Chromosoma 90:303–310
Lapitan NLV (1992) Organization and evolution of higher plant nuclear genomes. Genome 35:171–181
Lodhi MA, Reisch BI, Weeden NF (1993) Molecular genetic mapping of the Vitis genome (abstract). Am J Enol Vitic 44:469
Lodhi MA, Reisch BI, Weeden NF(1994) Genetic linkage maps of Vitis and QTL detection by interval mapping (abstract). Plant Genome II Conference, 24–27 January 1994 San Diego, California
Lukaszewski AJ, Wozna J, Apolinarska B, Naganowska B (1982) The DNA content of the individual chromosomes of rye. Theor Appl Genet 62:145–148
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
Martison VA (1975) Cytological studies of diploid and tetraploid Theobroma cacao. Genetica 45:341–348
Mukherjee S, Sharma AK (1990) Mitotic cell duration and its relationship with nuclear DNA content, chromosome number and size and rate of growth in species of Acacia growing under stress. Ind J Exp Biol 28:508–510
Mukherhee S, Sharma AK (1993) In situ nuclear DNA content in perennial fast and slow growing Acacias from arid zones. Cytobios 75:33–36
Narayan RKJ (1991) Molecular organisation of the plant genome: its relation to structure, recombination and the evolution of chromosomes. J Genet 70:43–61
Narayan RKJ, Rees H (1976) Nuclear DNA variation in Lathyrus. Chromosoma 54:141–154
Narayan RKJ, Rees H (1977) Nuclear DNA divergence among Lathyrus species. Chromosoma 63:101–107
Nishikawa K, Futura Y, Endo H (1979) Consideration of chromosome evolution on the basis of nuclear DNA content and total chromosome length in Lycoris. Jpn J Genet 54:387–396
Parida A, Raina SN, Narayan RJK (1990) Quantitative DNA variation between and within chromosome complements of Vigna spp. (Fabaceae). Genetica 82:125–134
Poggio L, Hunzikar JH (1986) Nuclear DNA content variation in Bulnesia. J Hered 77:43–48
Price HJ (1976) Evolution of DNA content in higher plants. Bot Rev 42:27–52
Price HJ (1988) DNA variation among higher plants. Ann Mis Bot Gard 75:1248–1257
Price HJ, Chambers KL, Bachmann K, Riggs J (1983) Inheritance of nuclear 2C DNA content variation in intraspecific and interspecific hybrids of Microseris (Asteraceae). Am J Bot 70:1133–1138
Rajasekaran K, Mullins MG (1983) The origin of embryos and plantlets from cultured anthers of hybrid grapevines. Am J Enol Vitic 34:108–113
Rao VLK, Sharma AK (1987) Amount of DNA and genotype differences in Hordeum. Cytologia 52:593–598
Rayburn AL, Price HJ, Smith JD, Gold JR (1985) C-band heterochromatin and DNA content in Zea mays. Am J Bot 72:1610–1617
Rayburn AL, Auger JA, Benzinger EA, Hepburn AG (1989) Detection of intraspecific DNA content variation in Zea mays L. by flow cytometry. J Exp Bot 40:1179–1183
Rayburn AL, Biradar DP, Bullock DG, McMurphy LM (1993) Nuclear DNA content F1 hybrids of maize. Heredity 70:294–300
SAS Institute (1985) SAS/STAT Guide for personal computers. SAS Institute Inc., Cary, North Carolina, USA
Saurer W, Antcliff AJ (1969) Polyploid mutants in grapes. Hort-Science 4:226–227
Shetty BV (1959) Cytotaxonomical studies in Vitaceae. Biblio Genet XVIII:167–272
Sims LE, Price HJ (1985) Nuclear DNA content variation in Helianthus (Asteraceae). Am J Bot 72:1213–1219
Srivastava S, Lavania UC (1991) Evolutionary DNA variation in Papaver. Genome 34:763–768
Stein DB, Thompson WF, Belford HS (1979) Studies on DNA sequences in Osmundaceae. J Mol Evol 13:215–232
Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Tesniére CM, Romieu C, Vayda ME (1993) Changes in gene expression of grapes in response to hypoxia. Am J Enol Vitic 44:445–451
Thomas MR, Matsumoto S, Cain P, Scott NS (1993) Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theor Appl Genet 86:173–180
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Lodhi, M.A., Reisch, B.I. Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae. Theoret. Appl. Genetics 90, 11–16 (1995). https://doi.org/10.1007/BF00220990
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DOI: https://doi.org/10.1007/BF00220990