Abstract
Fertility restoration by dominant nuclear genes is essential for hybrid breeding based on cytoplasmic male sterility (CMS) to obtain heterotic effects and high seed yields. In sunflower, only the PET1 sterility inducing cytoplasm has been used in commercial hybrid breeding until now. This particular male sterility was derived from an interspecific hybrid Helianthus petiolaris × H. annuus. For the recent work we used the segregating population RHA325(CMS) × HA342, based on the PET1 cytoplasm. Molecular markers were mapped within 1.1 cM around the restoration locus Rf1. At the distal side, the marker OP-K13_454 mapped at a distance of 0.9 cM and E32M36-155R at 0.7 cM from Rf1. At the proximal side the markers E44M70-275A, E42M76-125A and E33M61-136R were mapped at 0.1, 0.2, and 0.3 cM from the restorer locus, respectively. These markers provide an excellent basis for a map based cloning approach and for marker-assisted sunflower breeding.
Similar content being viewed by others
References
Balk, J. & C.J. Leaver, 2001. The PET1-CMS mitochondrial mutation in sunflower is associated with premature programmed cell death and cytochrome c release. Plant Cell 13: 1803–1818.
Bentolila, S., A.A. Alfonso & M.R. Hanson, 2002. A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants. Proc Natl Acad Sci USA 99: 10887–10892.
Berry, S.T., R.A. Allen, S.R. Barnes & P.D.S. Caligari, 1994. Molecular marker analysis of Helianthus annuus L. 1. Restriction fragment length polymorphisms between inbred lines of cultivated sunflower. Theor Appl Genet 89: 435–441.
Brahm, L., T. Rocher & W. Friedt, 2000. PCR-based markers facilitating marker assisted selection in sunflower for resistance to downy mildew. Crop Sci 40: 676–682.
Brown, G.G., N. Formanova, H. Jin, R. Wargaschuk, C. Dendy, P. Patil, M. Laforest, J. Zhang, W.Y. Cheung & B.S. Landry, 2003. The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35: 262–272.
Cheres, M.T., J.F. Miller, J.M. Crane & S.J. Knapp, 2000. Genetic distances as a predictor of heterosis and hybrid performance within and between heterotic groups in sunflower. Theor Appl Genet 100: 889–894.
Cui, X., R.P. Wise & P.S. Schnable, 1996. The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize. Science 272: 1334–1335.
Doyle, J.J. & J.L. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
Gagliardi, D. & C.J. Leaver, 1999. Polyadenylation accelerates the degradation of the mitochondrial mRNA associated with cytoplasmic male sterility in sunflower. EMBO J 18: 3757–3766.
Gentzbittel, L., Y.X. Zhang, F. Vear, B. Griveau & P. Nicolas, 1994. RFLP studies of genetic relationships among inbred lines of the cultivated sunflower, Helianthus annuus L.: Evidence for distinct restorer and maintainer germplasm pools. Theor Appl Genet 89: 419–425.
Hongtrakul, V., G.M. Huestis & S. Knapp, 1997. Amplified fragment length polymorphism as a tool for DNA fingerprinting sunflower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95: 400–407.
Horn, R., R.H. Köhler & K. Zetsche, 1991. A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Mol Biol 7: 26–33.
Horn, R., J.E.G. Hustedt, A. Horstmeyer, J. Hahnen, K. Zetsche & W. Friedt, 1996. The CMS-associated 16 kDa protein encoded by orfH522 is also present in other male sterile cytoplasms of sunflower. Plant Mol Biol 30: 523–538.
Horn, R., 2002. Molecular diversity of male sterility inducing and male-fertile cytoplasms in the genus Helianthus. Theor Appl Genet 104: 562–570.
Horn, R., B. Kusterer, E. Lazarescu, M. Prüfe & W. Friedt, 2003. Molecular mapping of the Rf1 gene restoring pollen fertility in PET1-based F1 hybrids in sunflower (Helianthus annuus L.). Theor Appl Genet 106: 599–606.
Köhler, R.H., R. Horn, A. Lössl & K. Zetsche, 1991. Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene. Mol Gen Genet 227: 369–376.
Koizuka, N., R. Imai, H. Fuijimoto, T. Hayakawa, Y. Kimura, J. Kohno-Murase, T. Sakai, S. Kawasaki & J. Imamura, 2003. Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J 34: 407–415.
Korell, M., G. Mösges & W. Friedt, 1992. Construction of a sunflower pedigree map. Helia 15: 7–16.
Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann Eugen 112: 172–175.
Kräuter, R., A. Steinmetz & W. Friedt, 1991. Efficient interspecific hybridisation in the genus Helianthus via “embryo rescue” and characterization of the hybrids. Theor Appl Genet 82: 521–525.
Kusterer, B., M. Prüfe, E. Lazarescu, N. Özdemir, W. Friedt & R. Horn, 2002. Mapping of the restorer gene Rf1 in sunflower (Helianthus annuus). Helia 25: 41–46.
Lander, E.S., J. Green, J. Abrahamson, A. Batlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.
Laver, H.K., S.J. Reynolds, F. Moneger & C.J. Leaver, 1991. Mitochondrial genome oragnization and expression associated with cytoplasmic male sterility in sunflower (Helianthus annuus) Plant J 1: 185–193.
Leclercq, P. 1969. Une stérilité mâle chez le tournesol. Ann Amélior Plantes 19: 99–106.
Leclercq, P. 1984. Identification de gènes de restauration de fertilité sur cytoplasmes stérilisants chez le tournesol. Agronomie 4: 573–576.
Matsui, K., Y. Mano, S. Taketa, N. Kawada & T. Komatsuda, 2001. Molecular mapping of a fertility restoration locus (Rfm1) for cytoplasmic male sterility in barley (Hordeum vulgare L.). Theor Appl Genet 102: 477–482.
Monéger, F., C.J. Smart & C.J. Leaver, 1994. Nuclear restoration of cytoplasmic male sterility in sunflower is associated with tissue-specific regulation of a novel mitochondrial gene. EMBO J 13: 8–17.
Rachid Al-Chaarani, G., L. Roustaee, L. Gentzbittel, L. Mokrani, G. Barrault, G. Dechamp-Guillaume & A. Sarrafi, 2002. A QTL analysis of sunflower partial resistance to downy mildew (Plasmopara halstedii) and black stem (Phoma macdonaldii) by the use of recombinant inbred lines (RILs). Theor Appl Genet 104: 490–496.
Serieys, H. 1996. Identification, study and utilisation in breeding programs of new CMS sources. FAO progress report. Helia 19 (special issue): 144–158.
Siculella, L. & J.D. Palmer, 1988. Physical and gene organization of mitochondrial DNA in fertile and male-sterile sunflower. Nucleic Acids Res 16: 3787–3799.
Sobral, B.W.S. & R.J. Honeycutt, 1993. High output genetic mapping of polyploids using PCR-generated markers. Theor Appl Genet 86: 105–112.
Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.
Yu, J.K., S. Tang, M.B. Slabaugh, A. Heesacker, G. Cole, M. Herring, J. Soper, F. Han, W.C. Chu, D.M. Webb, L. Thompson, K.J. Edwards, S. Berry, A.J. Leon, M. Grondona, C. Olungu, N. Maes & S.J. Knapp, 2003. Towards a saturated molecular genetic linkage map for cultivated sunflower. Crop Sci 43: 367–387.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kusterer, B., Horn, R. & Friedt, W. Molecular mapping of the fertility restoration locus Rf1 in sunflower and development of diagnostic markers for the restorer gene. Euphytica 143, 35–42 (2005). https://doi.org/10.1007/s10681-005-1795-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-1795-9