Skip to main content
SpringerLink
Log in

Construction of a reference molecular linkage map of globe artichoke (Cynara cardunculus var. scolymus)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The genome organization of globe artichoke (Cynara cardunculus var. scolymus), unlike other species belonging to Asteraceae (=Compositae) family (i.e. sunflower, lettuce and chicory), remains largely unexplored. The species is highly heterozygous and suffers marked inbreeding depression when forced to self-fertilize. Thus a two-way pseudo-testcross represents the optimal strategy for linkage analysis. Here, we report linkage maps based on the progeny of a cross between globe artichoke (C. cardunculus var. scolymus) and cultivated cardoon (C. cardunculus var. altilis). The population was genotyped using a variety of PCR-based marker platforms, resulting in the identification of 708 testcross markers suitable for map construction. The male map consisted of 177 loci arranged in 17 major linkage groups, spanning 1,015.5 cM, while female map was built with 326 loci arranged into 20 major linkage groups, spanning 1,486.8 cM. The presence of 84 loci shared between these maps and those previously developed from a cross within globe artichoke allowed for map alignment and the definition of 17 homologous linkage groups, corresponding to the haploid number of the species. This will provide a favourable property for QTL scanning; furthermore, as 25 mapped markers (8%) correspond to coding regions, it has an additional value as functional map and might represent an important genetic tool for candidate gene studies in globe artichoke.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Acquadro A, Portis E, Lanteri S (2003) Isolation of microsatellite loci in artichoke (Cynara cardunculus L. var. scolymus). Mol Ecol Notes 3:37–39

    Article  CAS  Google Scholar 

  • Acquadro A, Portis E, Lee D, Donini P, Lanteri S (2005a) Development and characterization of microsatellite markers in Cynara cardunculus L. Genome 48:217–225

    CAS  PubMed  Google Scholar 

  • Acquadro A, Portis E, Albertini E, Lanteri S (2005b) M-AFLP-based protocol for microsatellite loci isolation in Cynara cardunculus L. (Asteraceae). Mol Ecol Notes 5:272–274

    Article  CAS  Google Scholar 

  • Acquadro A, Portis E, Moglia A, Magurno F, Lanteri S (2006) Retrotransposon-based S-SAP as a platform for the analysis of genetic variation and linkage in globe artichoke. Genome 49:1149–1159

    Article  CAS  PubMed  Google Scholar 

  • Acquadro A, Lanteri S, Scaglione D, Arens P, Vosman B, Portis E (2009) Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke. Theor Appl Genet 118:1573–1587

    Article  CAS  PubMed  Google Scholar 

  • Bassam BJ, Caetanoanolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83

    Article  CAS  PubMed  Google Scholar 

  • Cervera MT, Storme V, Ivens B, Gusmao J, Liu BH, Hostyn V, Van Slycken J, Van Montagu M, Boerjan W (2001) Dense genetic linkage maps of three Populus species (Populus deltoides, P. nigra and P. trichocarpa) based on AFLP and microsatellite markers. Genetics 158:787–809

    CAS  PubMed  Google Scholar 

  • Chatelet P, Stamigna C, Thomas G (2005) Early development from isolated microspores of Cynara cardunculus var scolymus (L.) Fiori. Acta Hort 681:375–380

    Google Scholar 

  • Chiapparino E, Lee D, Donini P (2004) Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR. Genome 47:414–420

    Article  CAS  PubMed  Google Scholar 

  • Comino C, Lanteri S, Portis E, Acquadro A, Romani A, Hehn A, Larbat R, Bourgaud F (2007) Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L. BMC Plant Biol 7:14

    Article  PubMed  CAS  Google Scholar 

  • Comino C, Hehn A, Moglia A, Menin B, Bourgaud F, Lanteri S, Portis E (2009) The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway. BMC Plant Biol 9:30

    Article  PubMed  CAS  Google Scholar 

  • Costa P, Pot D, Dubos C, Frigerio JM, Pionneau C, Bodenes C, Bertocchi E, Cervera MT, Remington DL, Plomion C (2000) A genetic map of Maritime pine based on AFLP, RAPD and protein markers. Theor Appl Genet 100:39–48

    Article  CAS  Google Scholar 

  • Echt CS, Nelson CD (1997) Linkage mapping and genome length in eastern white pine (Pinus strobus L.). Theor Appl Genet 94:1031–1037

    Article  CAS  Google Scholar 

  • Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arus P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811

    Article  CAS  PubMed  Google Scholar 

  • Grandillo S, Tanksley SD (1996) Genetic analysis of RFLPs, GATA microsatellites and RAPDs in a cross between L. esculentum and L. pimpinellifolium. Theor Appl Genet 92:957–965

    Article  CAS  Google Scholar 

  • Hackett CA, Broadfoot LB (2003) Effects of genotyping errors, missing values and segregation distortion in molecular marker data on the construction of linkage maps. Heredity 90:33–38

    Article  CAS  PubMed  Google Scholar 

  • Hurtado MA, Romero C, Vilanova S, Abbott AG, Llacer G, Badenes ML (2002) Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.), and mapping of PPV (sharka) resistance. Theor Appl Genet 105:182–191

    Article  CAS  PubMed  Google Scholar 

  • Igarashi M, Abe Y, Hatsuyama Y, Ueda T, Fukasawa-Akada T, Kon T, Kudo T, Sato T, Suzuki M (2008) Linkage maps of the apple (Malus × domestica Borkh.) cvs ‘Ralls Janet’ and ‘Delicious’ include newly developed EST markers. Mol Breed 22:95–118

    Article  CAS  Google Scholar 

  • Jackson JA, Matthews D (2000) Modified inter-simple sequence repeat PCR protocol for use in conjunction with the LI-COR gene ImagIR(2) DNA analyzer. Biotechiques 28:914–917

    CAS  Google Scholar 

  • Jeuken M, van Wijk R, Peleman J, Lindhout P (2001) An integrated interspecific AFLP map of lettuce (Lactuca) based on two L-sativa × L-saligna F2 populations. Theor Appl Genet 103:638–647

    Article  CAS  Google Scholar 

  • Kenis K, Keulemans J (2005) Genetic linkage maps of two apple cultivars (Malus × domestica Borkh.) based on AFLP and microsatellite markers. Mol Breed 15:205–219

    Article  CAS  Google Scholar 

  • Krutovskii KV, Vollmer SS, Sorensen FC, Adams WT, Knapp SJ, Strauss SH (1998) RAPD genome maps of Douglas-fir. J Hered 89:197–205

    Article  CAS  Google Scholar 

  • Lanteri S, Portis E (2008) Globe artichoke and cardoon. In: Prohens J, Nuez F (eds) Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Springer, New York, pp 49–74

    Google Scholar 

  • Lanteri S, Di Leo I, Ledda L, Mameli M, Portis E (2001) RAPD variation within and among populations of globe artichoke cultivar ‘Spinoso sardo’. Plant Breed 120:243–246

    Article  CAS  Google Scholar 

  • Lanteri S, Saba E, Cadinu M, Mallica G, Baghino L, Portis E (2004) Amplified fragment length polymorphism for genetic diversity assessment in globe artichoke. Theor Appl Genet 108:1534–1544

    Article  CAS  PubMed  Google Scholar 

  • Lanteri S, Acquadro A, Comino C, Mauro R, Mauromicale G, Portis E (2006) A first linkage map of globe artichoke (Cynara cardunculus var. scolymus L.) based on AFLP, S-SAP, M-AFLP and microsatellite markers. Theor Appl Genet 112:1532–1542

    Article  CAS  PubMed  Google Scholar 

  • Laucou V, Haurogne K, Ellis N, Rameau C (1998) Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum. Theor Appl Genet 97:905–915

    Article  CAS  Google Scholar 

  • Lespinasse D, Rodier-Goud M, Grivet L, Leconte A, Legnate H, Seguin M (2000) A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsatellite, and isozyme markers. Theor Appl Genet 100:127–138

    Article  CAS  Google Scholar 

  • Lombard V, Delourme R (2001) A consensus linkage map for rapeseed (Brassica napus L.): construction and integration of three individual maps from DH populations. Theor Appl Genet 103:491–507

    Article  CAS  Google Scholar 

  • Lorieux M, Ndjiondjop MN, Ghesquiere A (2000) A first interspecific Oryza sativa × Oryza glaberrima microsatellite-based genetic linkage map. Theor Appl Genet 100:593–601

    CAS  Google Scholar 

  • Maliepaard C, Jansen J, Van Ooijen JW (1997) Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequences for applications. Genet Res 70:237–250

    Article  Google Scholar 

  • Maliepaard C, Alston FH, van Arkel G, Brown LM, Chevreau E, Dunemann F, Evans KM, Gardiner S, Guilford P, van Heusden AW, Janse J, Laurens F, Lynn JR, Manganaris AG, den Nijs APM, Periam N, Rikkerink E, Roche P, Ryder C, Sansavini S, Schmidt H, Tartarini S, Verhaegh JJ, Vrielink-van Ginkel M, King GJ (1998) Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet 97:60–73

    Article  CAS  Google Scholar 

  • Marie D, Brown S (1993) A cytometric exercise in plant DNA histograms, with 2C-values for 70 species. Biol Cell 78:41–51

    Article  CAS  PubMed  Google Scholar 

  • Marino R, Ponnaiah M, Krajewski P, Frova C, Gianfranceschi L, Pe ME, Sari-Gorla M (2009) Addressing drought tolerance in maize by transcriptional profiling and mapping. Mol Gen Genomics 281:163–179

    Article  CAS  Google Scholar 

  • Moglia A, Comino C, Portis E, Acquadro A, De Vos R, Beekwilder J, Lanteri S (2009) Isolation and mapping of a C3′H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress. Plant Cell Rep 28:963–974

    Article  CAS  PubMed  Google Scholar 

  • Motzo R, Deidda M (1993) Anther and ovule culture in globe artichoke. J Genet Breed 47:263–266

    Google Scholar 

  • Nikaido A, Yoshimaru H, Tsumura Y, Suyama Y, Murai M, Nagasaka K (1999) Segregation distortion for AFLP markers in Cryptomeria japonica. Genes Genet Syst 74:55–59

    Article  CAS  Google Scholar 

  • Pelgas B, Bousquet J, Beauseigle S, Isabel N (2005) A composite linkage map from two crosses for the species complex Picea mariana × Picea rubens and analysis of synteny with other Pinaceae. Theor Appl Genet 111:1466–1488

    Article  CAS  PubMed  Google Scholar 

  • Portis E, Barchi L, Acquadro A, Macua JI, Lanteri S (2005) Genetic diversity assessment in cultivated cardoon by AFLP (amplified fragment length polymorphism) and microsatellite markers. Plant Breed 124:299–304

    Article  CAS  Google Scholar 

  • Sargent DJ, Clarke J, Simpson DW, Tobutt KR, Arus P, Monfort A, Vilanova S, Denoyes-Rothan B, Rousseau M, Folta KM, Bassil NV, Battey NH (2006) An enhanced microsatellite map of diploid Fragaria. Theor Appl Genet 112:1349–1359

    Article  CAS  PubMed  Google Scholar 

  • Sebastian RL, Howell EC, King GJ, Marshall DF, Kearsey MJ (2000) An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations. Theor Appl Genet 100:75–81

    Article  CAS  Google Scholar 

  • Sonnante G, Carluccio A, De Paolis A, Pignone D (2008) Identification of artichoke SSR markers: molecular variation and patterns of diversity in genetically cohesive taxa and wild allies. Genet Resour Crop Evol 55:1029–1046

    Article  CAS  Google Scholar 

  • Stamigna C, Saccardo F, Pandozy G, Ancora G, Crinò P (2005) In vitro mutagenesis of globe artichoke (cv Romanesco). Acta Hortic 681:403–410

    Google Scholar 

  • Tanksley S, Ganal M, Prince J, Devicente M, Bonierbale M, Broun P, Fulton T, Giovannoni J, Grandillo S, Martin G, Messeguer R, Miller J, Miller L, Paterson A, Pineda O, Roder M, Wing R, Wu W, Young N (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Van Ooijen J (2006) JoinMap® 4: software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V, Wageningen

    Google Scholar 

  • Verde I, Lauria M, Dettori M, Vendramin E, Balconi C, Micali S, Wang Y, Marrazzo MT, Cipriani G, Hartings H, Testolin R, Abbott AG, Motto M, Quarta R (2005) Microsatellite and AFLP markers in the Prunus persica [L. (Batsch)] × P-ferganensis BC(1) linkage map: saturation and coverage improvement. Theor Appl Genet 111:1013–1021

    Article  CAS  PubMed  Google Scholar 

  • Vezzulli S, Troggio M, Coppola G, Jermakow A, Cartwright D, Zharkikh A, Stefanini M, Grando MS, Viola R, Adam-Blondon AF, Thomas M, This P, Velasco R (2008) A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses, based on 283 SSR and 501 SNP-based markers. Theor Appl Genet 117:499–511

    Article  CAS  PubMed  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Vandelee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA-fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  CAS  PubMed  Google Scholar 

  • Vuylsteke M, Mank R, Antonise R, Bastiaans E, Senior ML, Stuber CW, Melchinger AE, Lubberstedt T, Xia XC, Stam P, Zabeau M, Kuiper M (1999) Two high-density AFLP (R) linkage maps of Zea mays L.: analysis of distribution of AFLP markers. Theor Appl Genet 99:921–935

    Article  CAS  Google Scholar 

  • Waugh R, McLean K, Flavell AJ, Pearce SR, Kumar A, Thomas BBT, Powell W (1997) Genetic distribution of Bare-1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP). Mol Gen Genet 253:687–694

    Article  CAS  PubMed  Google Scholar 

  • Weeden N (1994) Approaches to mapping in horticultural crops. In: Gressho P (ed) Plant genome analysis. CRC Press, Boca Raton, pp 57–68

    Google Scholar 

  • Ye S, Dhillon S, Ke X, Collins A, Day I (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res 29:e88

    Article  CAS  PubMed  Google Scholar 

  • Zraidi A, Stift G, Pachner M, Shojaeiyan A, Gong L, Lelley T (2007) A consensus map for Cucurbita pepo. Mol Breed 20:375–388

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Aniko Stagel, Dr. Lorenzo Barchi and Dr. Andrea Moglia for their technical assistance. This research was supported by MIPAAF (Ministero delle Politiche Agricole, Alimentari e Forestali - Italy) through the CarVarVi (“Valorizzazione di germoplasma di carciofo attraverso la costituzione varietale ed il risanamento da virus”) project.

Author information

Authors and Affiliations

  1. DIVAPRA Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095, Grugliasco (Turin), Italy

    E. Portis, A. Acquadro, D. Scaglione & S. Lanteri

  2. Dipartimento di Scienze Agronomiche, Agrochimiche e delle Produzioni Animali, sez. Scienze Agronomiche, University of Catania, via Valdisavoia 5, 95123, Catania, Italy

    G. Mauromicale & R. Mauro

Authors
  1. E. Portis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Mauromicale
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Mauro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Acquadro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Scaglione
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Lanteri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Lanteri.

Additional information

Communicated by M. Havey.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Portis, E., Mauromicale, G., Mauro, R. et al. Construction of a reference molecular linkage map of globe artichoke (Cynara cardunculus var. scolymus). Theor Appl Genet 120, 59–70 (2009). https://doi.org/10.1007/s00122-009-1159-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-009-1159-2

Keywords

Search

Navigation