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Isolation and mapping of a C3′H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress

  • Genetics and Genomics
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Abstract

Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3′-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed maps.

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Acknowledgments

The authors kindly thank Harry Jonker (PRI) for his excellent technical assistance. The authors kindly thank Dr. Ullmann (Université Louis Pasteur, Strasbourg) for providing the substrates of the reaction, p-coumaroylquinate and p-coumaroylshikimate and for critical reading of the paper. The authors kindly thank Dr Nelson for providing CYP number to the new gene. The authors kindly thank Prof G. Mauromicale and Dr R. Mauro for providing plant material. Andrea Moglia acknowledges MIUR, for its financial support. Jules Beekwilder was financially supported by the EU 6th Frame FLORA project (2005-FOOD-CT-01730). Ric C. H. De Vos acknowledges initial support from the Centre for BioSystems Genomics, an initiative under the auspices of the Netherlands Genomics Initiative (NGI/NWO).

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Authors and Affiliations

  1. DiVaPRA, Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095, Grugliasco (TO), Italy

    Andrea Moglia, Cinzia Comino, Ezio Portis, Alberto Acquadro & Sergio Lanteri

  2. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Ric C. H. De Vos & Jules Beekwilder

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  1. Andrea Moglia
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  2. Cinzia Comino
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  3. Ezio Portis
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  4. Alberto Acquadro
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  5. Ric C. H. De Vos
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  6. Jules Beekwilder
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  7. Sergio Lanteri
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Corresponding author

Correspondence to Sergio Lanteri.

Additional information

Communicated by H. Judelson.

Nucleotide sequence data reported are available in the GenBank database under accession number: FJ225121

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Moglia, A., Comino, C., Portis, E. et al. Isolation and mapping of a C3′H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress. Plant Cell Rep 28, 963–974 (2009). https://doi.org/10.1007/s00299-009-0695-1

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  • DOI: https://doi.org/10.1007/s00299-009-0695-1

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