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Microsatellite and AFLP markers in the Prunus persica [L. (Batsch)]×P. ferganensis BC1linkage map: saturation and coverage improvement

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Abstract

A set of 146 single sequence repeats (SSRs) and 14 amplified fragment length polymorphism (AFLP) primer combinations were used to enrich a previously developed linkage map obtained from a (Prunus persica×P. ferganensis)×P. persica BC1 progeny. Forty-one SSR primer pairs gave polymorphic patterns detecting 42 loci. The restriction/selective primer AFLP combinations produced a total of 79 segregating fragments. The resulting map is composed of 216 loci covering 665 cM with an average distance of 3.1 cM. Novel regions were covered by the newly mapped loci for a total of 159 cM. Eight linkage groups were assembled instead of the earlier 10 as two small groups (G1a and G8b), previously independent, were joined to their respective major groups (G1b and G8a). Several gaps were also reduced resulting in an improved saturation of the map. Twelve gaps ≥10 cm are still present. A comparative analysis against the Prunus reference map (71 anchor loci) pointed out an almost complete synteny and colinearity. Six loci were not syntenic and only two were not colinear. Genetic distances were significantly longer in our map than in the reference one.

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Acknowledgements

This research was jontly funded by Ministero dell’Università e della Ricerca Scientifica e Tecnologica (MURST) and Ministero per le Politiche Agricole e Forestali (MIPAF) via FIRS grants (“Qualifrape” n. 14; “Indicatori di qualità”).

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Author notes

  1. Y. Wang

    Present address: Department of Plant Breeding, Cornell University, Ithaca, NY, 14853, USA

Authors and Affiliations

  1. Istituto Sperimentale per la Frutticoltura (CRA), Via di Fioranello 52, Ciampino Aeroporto, 00040, Rome, Italy

    I. Verde, M. T. Dettori, E. Vendramin, S. Micali & R. Quarta

  2. Istituto Sperimentale per la Cerealicoltura (CRA), Via Stezzano 24, 24100, Bergamo, Italy

    M. Lauria, C. Balconi, H. Hartings & M. Motto

  3. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Y. Wang & A. G. Abbott

  4. Dipartimento di Scienze agrarie e ambientali, Università di Udine, Via delle Scienze 208, 33100, Udine, Italy

    M. T. Marrazzo, G. Cipriani & R. Testolin

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  1. I. Verde
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  2. M. Lauria
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Correspondence to I. Verde.

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Communicated by F. Salamini

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Verde, I., Lauria, M., Dettori, M.T. et al. Microsatellite and AFLP markers in the Prunus persica [L. (Batsch)]×P. ferganensis BC1linkage map: saturation and coverage improvement. Theor Appl Genet 111, 1013–1021 (2005). https://doi.org/10.1007/s00122-005-0006-3

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