Skip to main content
SpringerLink
Log in

Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene (Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

References

  • Beavis WD (1998) QTL analyses: power, precision, and accuracy. In: Patterson AH (ed) Molecular dissection of complex traits. CRC Press, Boca Raton

  • Becker J, Heun M (1995) Barley microsatellites: allele variation and mapping. Plant Mol Biol 27:835–845

    CAS  PubMed  Google Scholar 

  • Castro A, Corey A, Filichkin T, Hayes PM, Sandoval-Islas JS, Vivar HE (2000) Stripe rust resistance QTL pyramids in barley. In: Logue S (ed) Proc 7th Int Barley Genet Symp vol 2. Department of plant sciences, Waite campus, Adelaide University, Glen Osmond, pp 86–88

  • Castro A, Chen X, Hayes PM, Knapp SJ, Line RF, Toojinda T, Vivar H (2002a) Coincident QTL that determine seedling and adult plant resistance to stripe rust in barley. Crop Sci 42:1701–1708

    CAS  Google Scholar 

  • Castro A, Hayes PM, Fillichkin T, Rossi C (2002b) Update of barley stripe rust resistance QTL in the Calicuchima-sib × Bowman mapping population. Barley Genet Newslett 32:1–12

    Google Scholar 

  • Castro A, Chen XM, Hayes PM, Johnson M (2003) Pyramiding of quantitative trait locus (QTL) alleles determining resistance to Barley Stripe Rust: effects on seedling resistance. Crop Sci 43 (in press)

  • Chen FQ, Hayes PM (1989) A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture. Theor Appl Genet 77:701–704

    Google Scholar 

  • Chen FQ, Prehn D, Hayes PM, Mulroney D, Corey A, Vivar H (1994) Mapping genes for resistance to barley stripe rust (Puccinia striiformis f.sp. hordei). Theor Appl Genet 88:215–219

    CAS  Google Scholar 

  • Ellis JG, Lawrence GJ, Peacock WK, Pryor AJ (1998) Approaches to cloning plant genes conferring resistance to fungal pathogens. Annu Rev Phytopathol 26:245–263

    Article  Google Scholar 

  • Holloway J, Knapp S (1994) gmendel 3.0. Users guide. Knapps@css.orst.edu

  • Hayes PM, Castro A, Corey A, Fillichkin T, Johnson M, Rossi C, Sandoval S, Vales I, Vivar H, Von Zitzewitz J (2001) Collaborative stripe rust resistance gene mapping and deployment efforts. In: Vivar H, McNab A (eds) Breeding Barley New Millennium: Proc Int Symp. CIMMYT, Mexico DF, pp 47–60

  • Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaradivel N, Bennett J, Khush GS (1997) Pyramiding of bacterial blight resistance genes in rice:marker-assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320

    CAS  Google Scholar 

  • Johnson R (1981) Durable resistance: definition of, genetic control, and attainment. Phytopathology 71:567–568

    Google Scholar 

  • Johnson R (1992) Past, present and future opportunities in breeding for disease resistance, with examples from wheat. Euphytica 63:3–22

    Google Scholar 

  • Kanazin V, Marex LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11,746–11,750

    Article  Google Scholar 

  • Kleinhofs A, Kilian A, Saghai Maroof M, Biyashev R, Hayes PM, Chen F, Lapitan N, Fenwick A, Blake T, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp S, Liu B, Sorrells M, Heun M, Franckowiak J, Hoffman D, Skadsen R, Steffenson B (1993) A molecular, isozyme, and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712

    CAS  Google Scholar 

  • Mano Y, Sayed-Tabatabaei BE, Graner A, Blake T, Takaiwa F, Oka S, Komatsuda T (1999) Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.). Theor Appl Genet 98:937–946

    CAS  Google Scholar 

  • McIntosh RA, Brown GN (1997) Anticipatory breeding for resistance to rust diseases in wheat. Annu Rev Phytopathol 35:311–326

    Article  CAS  Google Scholar 

  • Michelmore R (1995) Molecular approaches to manipulation of disease resistance genes. Annu Rev Phytopathol 15:393–427

    Article  Google Scholar 

  • Michelmore RW, Paran I, Vasili RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    PubMed  Google Scholar 

  • Mundt CC (1991) Probability of mutation to multiple virulence and durability of resistance gene pyramids: further comments. Phytopathology 81:240–242

    Google Scholar 

  • Liu Z-W, Biyashev RM, Saghai Maroof MA (1996) Development of simple sequence repeat markers and their integration into a barley linkage map. Theor Appl Genet 93:869–876

    CAS  Google Scholar 

  • Lynch M, Walsh B (1997) Genetics and analysis of quantitative traits. Sinauer, Sunderland, Mass.

  • Parlevliet JE (1977) Evidence of differential interaction in the polygenic Hordeum vulgare–Puccinia hordei relation during epidemic development. Phytopathology 67:776–778

    Google Scholar 

  • Parlevliet JE (1983) Race-specific resistance and cultivar-specific virulence in the barley-leaf rust pathosystem and their consequences for the breeding of leaf rust resistant barley. Euphytica 32:367–375

    Google Scholar 

  • Parlevliet JE (1989) Identification and evaluation of quantitative resistance. In: Leonard KJ, Fry WE (eds) Plant disease epidemiology, vol. 2. McGraw-Hill, New York, pp 215–248

  • Ramsay L, Macaulay M, degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuveson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    CAS  PubMed  Google Scholar 

  • Russell J, Fuller J, Young G, Thomas B, Taramino G, Macaulay M, Waugh R, Powell W (1997) Discriminating between barley genotypes using microsatellite markers. Genome 40:442–450

    CAS  PubMed  Google Scholar 

  • SAS (2001) Statistical analysis system online documentation. SAS Institute, Cary, N.C.

  • Sandoval-Islas JS, Broers LHM, Vivar H, Osada KS (1998) Evaluation of quantitative resistance to yellow rust (Puccinia striiformis f.sp. hordei) in the ICARDA/CIMMYT barley breeding program. Plant Breed 117:127–130

    Google Scholar 

  • Schafer JF, Roelfs AP (1985) Estimated relationship between numbers of urediniospores of Puccinia graminis f.sp. tritici and rates of occurrence of virulence. Phytopathology 75:749–750

    Google Scholar 

  • Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, Dhaliwal HS, Khush GS (2001) Pyramiding three bacterial blight resistance genes (xa5, xa13, Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet 102:1011–1015

    CAS  Google Scholar 

  • Thomas WTB, Powell W, Waugh R, Chalmers KJ, Barua UM, Jack P, Lea V, Forster BP, Swanston JS, Ellis RP, Hanson PR, Lance RCM (1995) Detection of quantitative trait loci for agronomic, yield, grain and disease characters in spring barley (Hordeum vulgare L.). Theor Appl Genet 91:1037–1047

    CAS  Google Scholar 

  • Toojinda T, Broers LH, Chen XM, Hayes PM, Kleinhofs A, Korte J, Kudrna D, Leung H, Line RF, Powell W, Ramsay L, Vivar H, Waugh R (2000) Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). Theor Appl Genet 101:580–589

    CAS  Google Scholar 

  • Vales I, Hayes PM, Castro A, Corey A, Mundt C, Capettini F, Vivar H, Sandoval-Islas S, Schoen C (2002) Resistance to barley stripe rust: a model for understanding genetically complex traits. In: Plant Anim Genome-10, Abstr, p 178 http://www.intl-pag.org/pag/10

  • Vanderplank JE (1963) Plant diseases: epidemics and control. Academic Press, New York

    Google Scholar 

  • Vanderplank JE (1978) Genetic and molecular basis of plant pathogenesis. Springer, Berlin, Heidelberg, New York

  • von Wettstein-Knowles P (1992) Cloned and mapped genes: current status. In: Shewry PR (ed) Barley: genetics, biochemistry, molecular biology and biotechnology. CAB Int, Wallingford, UK, pp 73–98

Download references

Acknowledgements

This research was supported by the North American Barley Genome Project, ICARDA/CIMMYT, American Malting Barley Association, Oregon Grains Commission, Idaho Barley Commission, and the Washington Barley Commission.

Author information

Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002, USA

    A. E. Corey, T. Filichkina, P. M. Hayes, K. Richardson & C. Rossi

  2. Departamento de Producción Vegetal, Est. Exp. "Dr. Mario A. Cassinoni", Facultad de Agronomía, Universidad de la República, Ruta 3 Km.373, Paysandú 60000, Uruguay

    A. J. Castro

  3. ICARDA/CIMMYT, Apdo. 6-641, 06600 Mexico, DF, Mexico

    F. Capettini & H. Vivar

  4. Department of Crop and Soil Sciences, Washington State University, 259 Johnson Hall, P.O. Box 646420, Pullman, WA 99164-6420, USA

    A. Kleinhofs

  5. Instituto de Fitosanidad, Colegio de Postgraduados, Montecillo, México, 56230

    S. Sandoval-Islas

  6. Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721-0036 USA

    D. Kudrna

Authors
  1. A. J. Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Capettini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. E. Corey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Filichkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. M. Hayes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Kleinhofs
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. Kudrna
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K. Richardson
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. S. Sandoval-Islas
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. C. Rossi
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. H. Vivar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. M. Hayes.

Additional information

Communicated by J.W. Snape

Oregon Agricultural Experiment Station paper No. 11953

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castro, A.J., Capettini, F., Corey, A.E. et al. Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. Theor Appl Genet 107, 922–930 (2003). https://doi.org/10.1007/s00122-003-1329-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1329-6

Keywords

Search

Navigation