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Agrobacterium rhizogenes-Mediated Transformation in Medicinal Plants: Genetic Stability in Long-Term Culture

  • Living reference work entry
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Transgenesis and Secondary Metabolism

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Variations at morphological, cytogenetical, cytochemical, biochemical, and molecular levels have been reported in cell, callus cultures, clonally propagated plants, and in regenerated plants in some plant species. Metabolic instability in the genetically manipulated transgenic cell lines with respect to secondary metabolite production has been reported by different authors in long-term in vitro culture, although in a few cases the transgenic nature of the cell lines was retained. Transgenic hairy root cultures are another promising way of production of commercially valuable secondary metabolites in vitro, which open up a new dimension of the role of plant tissue culture in secondary metabolite production. Hairy root cultures and the plants regenerated from transformed roots are well known for their cytogenetical, morphological, and biochemical stability when compared to cell suspension cultures and callus cultures. But there are very few studies on the stability of hairy roots under long-term cultural condition. Variability in Ri-transformed root cultures and regenerated Ri-transformed plants has also been reported in a few species. In the present review, the stability/instability of hairy root cultures and Ri-plants maintained in vitro for long term is discussed in detail.

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Authors and Affiliations

  1. Department of Botany, University of Calcutta, 35 Ballygunge circular Road, 700019, Kolkata, India

    Dipasree Roychowdhury, Mihir Halder & Sumita Jha

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  1. Dipasree Roychowdhury
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  2. Mihir Halder
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  3. Sumita Jha
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Correspondence to Dipasree Roychowdhury , Mihir Halder or Sumita Jha .

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  1. Department of Botany, Calcutta University, Kolkata, West Bengal, India

    Sumita Jha

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Roychowdhury, D., Halder, M., Jha, S. (2016). Agrobacterium rhizogenes-Mediated Transformation in Medicinal Plants: Genetic Stability in Long-Term Culture. In: Jha, S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-27490-4_8-1

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  • DOI: https://doi.org/10.1007/978-3-319-27490-4_8-1

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  • Online ISBN: 978-3-319-27490-4

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