Abstract
We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350–510% in the roots and 200–320% in the whole plants) and augmented tylophorine content (20–60%) in the shoots, resulting in a 160–280% increase in tylophorine production in different clones grown in vitro.
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Abbreviations
- BA:
-
6-Benzyl adenine
- BM:
-
Basal medium
- HPLC:
-
High performance liquid chromatography
- Ri:
-
Root-inducing
- T-DNA:
-
Transferred DNA
- TL-DNA:
-
Left-handed T-DNA
- WT:
-
Wild type
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Acknowledgements
We thank the Programme Coordinator, Centre of Advanced Study, Department of Botany, University of Calcutta for the facilities provided, Dr. F. Abe, Fukuoka University, Japan for the standard tylophorine samples, Dr. A. Kollman, INRA, Versailles, France for his support in tylophorine analysis and Mr. N. Roy for photography. This work was funded by the Indo-French Centre for the Promotion of Advanced Research (Centre Franco-Indien pour la Promotion de la Recherche Avancée) and the Department of Science and Technology, Government of India.
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Chaudhuri, K.N., Ghosh, B., Tepfer, D. et al. Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes . Plant Cell Rep 25, 1059–1066 (2006). https://doi.org/10.1007/s00299-006-0164-z
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DOI: https://doi.org/10.1007/s00299-006-0164-z