Abstract
Belgian endive (Cichorium intybus) was genetically transformed usingAgrobacterium rhizogenes to insert wild type root-inducing, leftward, transferred DNA (Ri TL-DNA) into the nuclear genome. Transformed root cultures gave rise to plants (R0 generation) having the transformed phenotype described for other species, including increased branching, sterility, annual flowering and wrinkled leaves. Transformation circumvented the need for vernalization in order to flower, but not the need for inductive day length. Progeny (R1 generation) were analysed by molecular hybridization and phenotypes were characterized relative to normal controls and to the R0 generation. The extent of the T l -DNA varied among siblings, with restriction fragmentEco R1 15, containing open reading frames 10, 11 and 12 (rol A, B and C), segregating as a single insertion. Phenotypic alterations in these plants indicate that the transformed phenotype in endive is at least partially due to the genes carried on theEco R1 fragment 15.
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Sun, LY., Touraud, G., Charbonnier, C. et al. Modification of phenotype in Belgian endive (Cichorium intybus) through genetic transformation byAgrobacterium rhizogenes: conversion from biennial to annual flowering. Transgenic Research 1, 14–22 (1991). https://doi.org/10.1007/BF02512992
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DOI: https://doi.org/10.1007/BF02512992