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Somatic Embryogenesis in Olive (Olea europaea L. subsp. europaea var. sativa and var. sylvestris)

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

Protocols for olive somatic embryogenesis from zygotic embryos and mature tissues have been described for both Olea europaea sub. europaea var. sativa and var. sylvestris. Immature zygotic embryos (no more than 75 days old), used after fruit collection or stored at 12–14 °C for 2–3 months, are the best responsive explants and very slightly genotype dependent, and one single protocol can be effective for a wide range of genotypes. On the contrary, protocols for mature zygotic embryos and for mature tissue of cultivars are often genotype specific, so that they may require many adjustments according to genotypes. The use of thidiazuron and cefotaxime seems to be an important trigger for induction phase particularly for tissues derived from cultivars. Up to now, however, the application of this technique for large-scale propagation is hampered also by the low rate of embryo germination; it proves nonetheless very useful for genetic improvement.

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Authors and Affiliations

  1. Department of Agriculture, Forests, Nature and Energy (DAFNE), University of Tuscia, Via San Camillo De Lellis snc, 01100, Viterbo, Italy

    Eddo Rugini & Cristian Silvestri

Authors
  1. Eddo Rugini
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  2. Cristian Silvestri
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Correspondence to Eddo Rugini .

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Editors and Affiliations

  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Rugini, E., Silvestri, C. (2016). Somatic Embryogenesis in Olive (Olea europaea L. subsp. europaea var. sativa and var. sylvestris). In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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