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Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

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Abstract

The crop species Olea europaea L. (olive tree) is of great economic importance in the Mediterranean region. Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods. On the other hand, the lesser known Olea maderensis (Lowe) Rivas Mart. & Del Arco which is a native species of the Madeira Archipelago has only been the subject of micropropagation from nodal stem cuttings. Therefore, in this work we analysed the stability of ten nuclear simple sequence repeat (SSR) markers at successive stages of the somatic embryogenesis process in two adult trees belonging to these two species from the Madeira Archipelago. For the induction of somatic embryogenesis, petiole and leaf explants were cultivated on solid Murashige and Skoog medium (MS) with 12.25 μM indole-3-butyric acid (IBA) and 4.56 μM of zeatin, in the dark. After 3 months, different callus tissues (non-embryogenic, pre-embryogenic and embryogenic) developed from leaf explants and petioles were later transferred to MS medium without growth regulators in the dark. All ten SSR markers were able to distinguish between species. However, no mutations were found at the SSR loci at any of the successive developmental stages from PEMs (pre-embryogenic masses) to somatic embryos. This genetic uniformity was observed within material derived from each genotype/species and its respective donor plant. Therefore, we conclude that the genomic integrities of both O. europaea and O. maderensis were maintained throughout the stages of the embryogenic processes in study suggesting the absence of somaclonal variation.

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Acknowledgments

The authors thank Eduardo Ferreira and Luis Souto for technical assistance in the analyses of fragments. Thanks are also due to Armando Costa, Raquel Santos and Ricardo Cruz. This work was supported by the FCT/MCTES project POCI/AGR/60672/2004. FCT also supported the fellowship of Tina Lopes (FCT/SFRH/BPD/6012/2001).

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  1. Laboratory of Biotechnology and Cytomics, CESAM and Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Tina Lopes, Ana Capelo, Gina Brito, João Loureiro & Conceição Santos

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  1. Tina Lopes
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  2. Ana Capelo
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  3. Gina Brito
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  4. João Loureiro
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Correspondence to Tina Lopes.

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Communicated by M. Canovas.

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Lopes, T., Capelo, A., Brito, G. et al. Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites. Trees 23, 29–36 (2009). https://doi.org/10.1007/s00468-008-0251-6

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