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Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) ‘Chetoui’

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Abstract

Somatic embryogenesis of olive Olea europaea (L.) ‘Chetoui’ was studied using cell suspension cultures initiated from mature leaf-derived calli. Calli were developed on half-strength MS medium supplemented with 10 μM NAA and 2.25 μM 2i-P in the dark. Different combinations of three plant growth regulators (2,4-D, NAA and zeatin) were tested to determine cell proliferation and somatic embryogenesis induction and differentiation. Embryogenic suspension cultures were established in olive-modified medium for embryogenesis (OMe) containing 2.5 μM 2,4-D and 2.5 μM zeatin. Pre-globular and globular embryos were induced from mature olive tissue in liquid medium. In addition, the nitrogen form as inorganic (reduced; (NH4)2SO4 or oxidized; KNO3) and organic (CH) was used separately or in combination to improve the cell growth and proliferation. The most effective growth rate and cell proliferation were obtained with the medium containing inorganic and organic nitrogen forms.

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Abbreviations

2i-P:

Isopentenyl adenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

Naphthaleneacetic acid

OM:

Olive medium (Rugini 1984)

OMc:

Olive-modified medium (Canas and Benbadis 1988)

OMe:

Olive-modified medium (Rugini and Caricato 1995)

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Acknowledgments

We are most grateful to anonymous reviewers for the very useful comments in the improvement of the manuscript. Thanks are also due to Drs M. Ben Naceur, A. Ben Abdallah and A. Jemmali for the helpful discussion on somatic embryogenesis in woody plants. We also gratefully acknowledge the assistance of the technical staff of the Laboratory of Biotechnology and Plant Physiology in the National Institute of Agronomical Research of Tunisia and the National Institute of Olive of Tunisia.

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  1. El Bahri Trabelsi

    Present address: , BP no. 2, 2040, Rades, Tunisia

Authors and Affiliations

  1. Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis, Campus Universitaire, 1060, Tunis, Tunisia

    El Bahri Trabelsi, Selima Naija & Sadok Bouzid

  2. Institut de l’Olivier de Tunisie, Rue Hedi Karray, 2049, Ariana, Tunisia

    Monji Msellem

  3. Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049, Ariana, Tunisia

    El Bahri Trabelsi, Selima Naija, Nedra Elloumi, Zina Belfeleh & Rachida Ghezel

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  1. El Bahri Trabelsi
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  2. Selima Naija
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  3. Nedra Elloumi
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Correspondence to El Bahri Trabelsi.

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Communicated by S. Werbrouck.

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Trabelsi, E.B., Naija, S., Elloumi, N. et al. Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) ‘Chetoui’. Acta Physiol Plant 33, 319–324 (2011). https://doi.org/10.1007/s11738-010-0550-6

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  • DOI: https://doi.org/10.1007/s11738-010-0550-6

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