Abstract
In calli from distal and proximal cotyledon segments and from radicles of mature wild olive zygotic embryos, rhizogenesis prevailed during the first and somatic embryogenesis during the second 30 day subculture period. Rhizogenesis was enhanced by low IBA (0.5 and 2.5 μM) and 5.0 μM 2iP. By reducing by half the media salt concentrations and by inserting a 21 day dark period rhizogenesis was also enhanced. Somatic embryogenesis was inhibited by both 2iP and IBA at concentrations higher than 5.0 μM, but was not influenced by lower salt concentrations or by inserting the dark period.
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Abbreviations
- 2.4-D:
-
2.4-dichlorophenoxyacetic acid
- 2iP:
-
N6-[2-isopentenyl]adenine
- BA:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige & Skoog
- NAA:
-
naphthaleneacetic acid
- OM:
-
Olive medium [4]
- OMc:
-
OM medium in which OM macroelements were replaced with the ones proposed by Bourgin & Nitsch [2]
- OMe:
-
OM salts of quarter strength, with half strength vitamin mixture (except biotin which added in full strength), with 5 μM 2iP and 20 g l-1 sucrose [2]
- OMr:
-
OM salts half strength containing 5 μM IBA and 20 g l-1 sucrose [2]
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Orinos, T., Mitrakos, K. Rhizogenesis and somatic embryogenesis in calli from wild olive (Olea europaea var. sylvestris (Miller) Lehr) mature zygotic embryos. Plant Cell Tiss Organ Cult 27, 183–187 (1991). https://doi.org/10.1007/BF00041288
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DOI: https://doi.org/10.1007/BF00041288