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Electroporation Transformation of Barley

  • Chapter
Genetic Transformation of Plants

Part of the book series: Molecular Methods of Plant Analysis ((MOLMETHPLANT,volume 23))

Abstract

Barley (Hordeum vulgare L.) is a member of the tribe Triticeae of the family Poaceae (von Bothmer et al. 1991), which was widely adapted to temperate regions of the world. Barley has both two- and six-rowed types according to spike morphology; intermediate types also exist. Due to its good adaptation to unfavorable climates, barley is produced in a significant quantity and consumed as livestock feed, food for man and malt brewery materials. In 2001, barley was harvested from 53,827,895 ha (FAO, http://apps.fao.org/page/collections) and yielded 25,723 hg/ha of grain in the world. Today, barley is not only the fourth most important cereal crop (after maize, wheat and rice) in the world, but also an excellent model plant for biochemists, physiologists, geneticists and molecular biologists (Shewry 1992) because of its inbred diploid nature, low chromosome number 2n = 2x = 14 (von Bothmer 1992) and relatively small genome size. The barley genome contains nearly 5.5 pg of DNA per haploid nucleus, equivalent to approximately 5.3 x 109bp (Bennet and Smith 1976). Ease of growth under laboratory conditions facilitates the development of molecular markers for construction of genetic maps (Becker and Heun 1995; Hayes et al. 1996; Manninen 2000; Ramsay et al. 2000; Williams et al. 2001), genome analysis (Michalek et al. 2002) and functional genomics studies (Öztürk et al. 2002; Sreenivasulu et al. 2002). Special attention has also been given to the establishment of tissue culture and development of gene transfer systems in order to obtain desirable genotypes (Lemaux et al. 1999).

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  1. F. Gürel
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  1. Department of Horticulture, Viticulture and Oenology, University of Adelaide, Waite Campus, 5064, Glen Osmond, SA, Australia

    J. F. Jackson

  2. Goldberglein 7, 91056, Erlangen, Germany

    H. F. Linskens

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Gürel, F., Gözükirmizi, N. (2003). Electroporation Transformation of Barley. In: Jackson, J.F., Linskens, H.F. (eds) Genetic Transformation of Plants. Molecular Methods of Plant Analysis, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07424-4_5

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  • DOI: https://doi.org/10.1007/978-3-662-07424-4_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-05553-9

  • Online ISBN: 978-3-662-07424-4

  • eBook Packages: Springer Book Archive

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