Abstract
Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.
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Laursen, C.M., Krzyzek, R.A., Flick, C.E. et al. Production of fertile transgenic maize by electroporation of suspension culture cells. Plant Mol Biol 24, 51–61 (1994). https://doi.org/10.1007/BF00040573
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DOI: https://doi.org/10.1007/BF00040573