Summary
We have developed a method for reproducibly obtaining transgenic rice at a high frequency (10-4): electroporation with a buffer in which chloride ions are replaced with organic acids. Co-transformation frequencies of the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes located on two separate plasmids were higher than 50%. Transgenic rice plants contained multiple copies of introduced genes integrated into their genomes in a complex manner. GUS enzyme activity was not proportional to gene copy number. Introduced HPT genes were detected and expressed in the progeny of transformants.
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Tada, Y., Sakamoto, M. & Fujimura, T. Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions. Theoret. Appl. Genetics 80, 475–480 (1990). https://doi.org/10.1007/BF00226748
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DOI: https://doi.org/10.1007/BF00226748