Gel-Free Proteomics

Methods and Protocols

  • Kris Gevaert
  • Joël Vandekerckhove

Part of the Methods in Molecular Biology book series (MIMB, volume 753)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Kenny Helsens, Lennart Martens, Joël Vandekerckhove, Kris Gevaert
    Pages 1-27
  3. Joost W. Gouw, Bastiaan B.J. Tops, Jeroen Krijgsveld
    Pages 29-42
  4. Wei-Jun Qian, Brianne O. Petritis, Carrie D. Nicora, Richard D. Smith
    Pages 43-54
  5. Friedrich Lottspeich, Josef Kellermann
    Pages 55-64
  6. Magnus Ø. Arntzen, Christian J. Koehler, Achim Treumann, Bernd Thiede
    Pages 65-76
  7. Casey J. Krusemark, Brian L. Frey, Lloyd M. Smith, Peter J. Belshaw
    Pages 77-91
  8. Dorothée Lebert, Alain Dupuis, Jérôme Garin, Christophe Bruley, Virginie Brun
    Pages 93-115
  9. Sophie Duclos, Michel Desjardins
    Pages 117-128
  10. Ping Sui, Tasso Miliotis, Max Davidson, Roger Karlsson, Anders Karlsson
    Pages 129-142
  11. Gertjan Kramer, Piotr T. Kasper, Luitzen de Jong, Chris G. de Koster
    Pages 169-181
  12. Heidi Rosenqvist, Juanying Ye, Ole N. Jensen
    Pages 183-213
  13. Martijn W. H. Pinkse, Simone Lemeer, Albert J. R. Heck
    Pages 215-228
  14. Gemma R. Davidson, Stuart D. Armstrong, Robert J. Beynon
    Pages 229-242
  15. Oliver Schilling, Ulrich auf dem Keller, Christopher M. Overall
    Pages 257-272
  16. Alain Doucet, Oded Kleifeld, Jayachandran N. Kizhakkedathu, Christopher M. Overall
    Pages 273-287
  17. Els J. M. Van Damme
    Pages 289-297
  18. Katharina Lohrig, Albert Sickmann, Urs Lewandrowski
    Pages 299-308
  19. Giuseppe Palmisano, Sara E. Lendal, Martin R. Larsen
    Pages 309-322
  20. Yelena Kravtsova-Ivantsiv, Aaron Ciechanover
    Pages 335-357
  21. Niklaas Colaert, Joël Vandekerckhove, Lennart Martens, Kris Gevaert
    Pages 373-398
  22. Back Matter
    Pages 399-400

About this book


Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this critical field.


LPI hexalene glycosylated proteins isobaric peptide termini labeling (IPTL) mass spectrometry organelle proteomics phosphoproteomics proteome research

Editors and affiliations

  • Kris Gevaert
    • 1
  • Joël Vandekerckhove
    • 2
  1. 1., VIB Department of Medical Protein ResearVIB and Ghent UniversityGentBelgium
  2. 2., VIB Department of Medical Protein ResearVIB and Ghent UniversityGentBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-147-5
  • Online ISBN 978-1-61779-148-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site