Organelle Proteomics

  • Sophie Duclos
  • Michel Desjardins
Part of the Methods in Molecular Biology book series (MIMB, volume 753)


Proteomics has significantly contributed to improve our understanding of cell structures and functions in the last decade. The possibility to identify large sets of proteins from minute amount of material, linked with the isolation of cellular organelles using various cell fractionation methods, has provided unique insights into the molecular mechanisms governing cell functions in health and disease. The success of this approach relies on the isolation of highly enriched cell fractions enabling the separation of organelles with minimal contamination by other cellular structures.

Key words

Phagocytosis endocytosis plasma membrane cell fractionation centrifugation 



This work was supported by the Canadian Institute for Health Research and the Canadian Research Chairs.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Sophie Duclos
    • 1
  • Michel Desjardins
    • 2
  1. 1.Département de Pathologie et Biologie CellulaireUniversité de MontréalMontréalCanada
  2. 2.Département de Pathologie et Biologie Cellulaire, Département de microbiologie et immunologieUniversité de MontréalMontréalCanada

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