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Organelle Proteomics

  • Sophie Duclos
  • Michel Desjardins
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 753)

Abstract

Proteomics has significantly contributed to improve our understanding of cell structures and functions in the last decade. The possibility to identify large sets of proteins from minute amount of material, linked with the isolation of cellular organelles using various cell fractionation methods, has provided unique insights into the molecular mechanisms governing cell functions in health and disease. The success of this approach relies on the isolation of highly enriched cell fractions enabling the separation of organelles with minimal contamination by other cellular structures.

Key words

Phagocytosis endocytosis plasma membrane cell fractionation centrifugation 

Notes

Acknowledgments

This work was supported by the Canadian Institute for Health Research and the Canadian Research Chairs.

References

  1. 1.
    Brunet, S., Thibault, P., Gagnon, E., Kearney, P., Bergeron, J.J., and Desjardins, M. (2003) Organelle proteomics: looking at less to see more. Trends Cell Biol 13, 629–638.PubMedCrossRefGoogle Scholar
  2. 2.
    Au, C.E., Bell, A.W., Gilchrist, A., Hiding, J., Nilsson, T., and Bergeron, J.J. (2007) Organellar proteomics to create the cell map. Curr Opin Cell Biol 19, 376–385.PubMedCrossRefGoogle Scholar
  3. 3.
    Lanoix, J., Ouwendijk, J., Lin, C.C., Stark, A., Love, H.D., Ostermann, J., and Nilsson, T. (1999) GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles. EMBO J 18, 4935–4948.PubMedCrossRefGoogle Scholar
  4. 4.
    Sircar, K., Gaboury, L., Ouadi, L., Mecteau, M., Scarlata, E., Saad, F., Aprikian, A., Tanguay, S., Lapointe, S., Lussier, C., Miletti, T., and Lanoix, J. (2006) Isolation of human prostatic epithelial plasma membranes for proteomics using mirror image tissue banking of radical prostatectomy specimens. Clin Cancer Res 12, 4178–4184.PubMedCrossRefGoogle Scholar
  5. 5.
    Jutras, I., Houde, M., Currier, N., Boulais, J., Duclos, S., LaBoissière, S., Bonneil, E., Kearney, P., Thibault, P., Paramithiotis, E., Hugo, P., and Desjardins, M. (2008) Modulation of the phagosome proteome by interferon-gamma. Mol Cell Proteomics 7, 697–715.PubMedGoogle Scholar
  6. 6.
    Desjardins, M., Huber, L.A., Parton, R.G., and Griffiths, G. (1994) Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus. J Cell Biol 124, 677–688.PubMedCrossRefGoogle Scholar
  7. 7.
    Stuart, L.M., Boulais, J., Charriere, G.M., Hennessy, E.J., Brunet, S., Jutras, I., Goyette, G., Rondeau, C., Letarte, S., Huang, H., Ye, P., Morales, F., Kocks, C., Bader, J.S., Desjardins, M., and Ezekowitz, R.A. (2007) A systems biology analysis of the Drosophila phagosome. Nature 445, 95–101.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Sophie Duclos
    • 1
  • Michel Desjardins
    • 2
  1. 1.Département de Pathologie et Biologie CellulaireUniversité de MontréalMontréalCanada
  2. 2.Département de Pathologie et Biologie Cellulaire, Département de microbiologie et immunologieUniversité de MontréalMontréalCanada

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