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Embryo Models In Vitro

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Magdalena Zernicka-Goetz

    1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Kursad Turksen

    1. Ottawa, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2767)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Generation of Human Blastoids from Naive Pluripotent Stem Cells

    • Yulei Wei, Kun Liu, Carlos A. Pinzon-Arteaga, Deirdre Logsdon, Leqian Yu, Ye Yuan et al.
    Pages 1-18

  3. Human Pre-gastrulation Embryo Culture in 3D Condition

    • Lifeng Xiang, Yu Yin, Gaohui Shi, Tianqing Li
    Pages 19-25

  4. Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System

    • Yu-Xin Luo, Yang Yu
    Pages 27-41

  5. Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models

    • Hao Wu, Yue Wang, Hongmei Wang
    Pages 43-52

  6. Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology

    • Hao Wu, Yue Wang, Hongmei Wang
    Pages 53-62

  7. Endometrial Assembloids to Model Human Embryo Implantation In Vitro

    • Thomas M. Rawlings, Maria Tryfonos, Komal Makwana, Deborah M. Taylor, Jan J. Brosens, Emma S. Lucas
    Pages 63-74

  8. Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (μPASE)

    • Yi Zheng
    Pages 75-84

  9. Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells

    • Rowan M. Karvas, Thorold W. Theunissen
    Pages 85-103

  10. Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells

    • Amitesh Panda, Thi Xuan Ai Pham, Sherif Khodeer, Vincent Pasque
    Pages 105-113

  11. Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells

    • Yuchuan Miao, Margarete Diaz-Cuadros, Olivier Pourquié
    Pages 115-122

  12. Generation of Stem Cell-Based Mouse Embryo-Like Structures

    • Charlotte E. Handford, Pallavi Panda, Ikbal B. Mohammad Choudhury, Gianluca Amadei, Magdalena Zernicka-Goetz
    Pages 123-134

  13. Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond

    • Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh
    Pages 135-159

  14. Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts

    • Sharon Arcuri, Georgia Pennarossa, Sergio Ledda, Fulvio Gandolfi, Tiziana A. L. Brevini
    Pages 161-173

  15. Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast

    • Asha Shaji Antony, A. S. Devika, Smita Sudheer
    Pages 175-188

  16. Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos

    • Savana Biondic, Cheng Zhao, Michael Hagemann-Jensen, Stewart J. Russell, Katherine Vandal, Jesica Canizo et al.
    Pages 189-212

  17. Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas

    • San Kit To, Bradley Balaton, Vincent Pasque
    Pages 213-250

  18. Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL

    • Tiam Heydari, Peter W. Zandstra
    Pages 251-262

  19. ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis

    • Ceren Pajanoja, Laura Kerosuo
    Pages 263-273

  20. Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence

    • Jesica Canizo, Savana Biondic, Katherine Vandal Lenghan, Sophie Petropoulos
    Pages 275-292
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Keywords

About this book

This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Embryo Models In Vitro: Methods and Protocols serves as an ideal guide to these exciting and innovative advances in developmental biology.

Editors and Affiliations

  • Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, USA

    Magdalena Zernicka-Goetz

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Embryo Models In Vitro

  • Book Subtitle: Methods and Protocols

  • Editors: Magdalena Zernicka-Goetz, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3686-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3685-5Published: 07 February 2024

  • Softcover ISBN: 978-1-0716-3688-6Due: 09 March 2024

  • eBook ISBN: 978-1-0716-3686-2Published: 06 February 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 323

  • Number of Illustrations: 3 b/w illustrations

  • Topics: Developmental Biology, Regenerative Medicine/Tissue Engineering, Cell Biology

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