Abstract
Paraxial mesoderm in the early embryo is segmented into epithelial blocks called somites that establish the metameric organization of the vertebrate body plan. Somites are sequentially formed from head to tail in a rhythmic manner controlled by an oscillating gene regulatory network known as the segmentation clock. We know very little about this important process during human development due to limited access to human embryos and ethical concerns. To bypass these difficulties, model systems derived from human pluripotent stem cells have been established. Here, we detail three protocols modeling different aspects of human paraxial mesoderm development in vitro: a 2D cell monolayer system recapitulating dynamics of the human segmentation clock, a 3D organoid system called “somitoid” supporting the simultaneous formation of somite-like structures, and another organoid system called “segmentoid” reconstituting in vivo–like hallmarks of somitogenesis. Together, these complementary model systems provide an excellent platform to decode somitogenesis and advance human developmental biology.
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References
Hubaud A, Pourquié O (2014) Signalling dynamics in vertebrate segmentation. Nat Rev Mol Cell Biol 15:709–721
Eckalbar WL, Fisher RE, Rawls A, Kusumi K (2012) Scoliosis and segmentation defects of the vertebrae. Wiley Interdiscip Rev Dev Biol 1:401–423
Oates AC, Morelli LG, Ares S (2012) Patterning embryos with oscillations: structure, function and dynamics of the vertebrate segmentation clock. Development 139:625–639
Schoenwolf GC, Bleyl SB, Brauer PR, Francis-West PH (2020) Larsen’s human embryology E-book. Elsevier Health Sciences
Chu L-F, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA (2019) An in vitro human segmentation clock model derived from embryonic stem cells. Cell Rep 28:2247–2255.e5
Diaz-Cuadros M, Wagner DE, Budjan C, Hubaud A, Tarazona OA, Donelly S, Michaut A, Al Tanoury Z, Yoshioka-Kobayashi K, Niino Y, Kageyama R, Miyawaki A, Touboul J, Pourquié O (2020) In vitro characterization of the human segmentation clock. Nature. https://doi.org/10.1038/s41586-019-1885-9
Matsuda M, Yamanaka Y, Uemura M, Osawa M, Saito MK, Nagahashi A, Nishio M, Guo L, Ikegawa S, Sakurai S, Kihara S, Maurissen TL, Nakamura M, Matsumoto T, Yoshitomi H, Ikeya M, Kawakami N, Yamamoto T, Woltjen K, Ebisuya M, Toguchida J, Alev C (2020) Recapitulating the human segmentation clock with pluripotent stem cells. Nature 580:124–129
Sanaki-Matsumiya M, Matsuda M, Gritti N, Nakaki F, Sharpe J, Trivedi V, Ebisuya M (2022) Periodic formation of epithelial somites from human pluripotent stem cells. Nat Commun 13:1–14
Miao Y, Djeffal Y, De Simone A, Zhu K, Lee JG, Lu Z, Silberfeld A, Rao J, Tarazona OA, Mongera A, Rigoni P, Diaz-Cuadros M, Song LMS, Di Talia S, Pourquié O (2022) Reconstruction and deconstruction of human somitogenesis in vitro. Nature. https://doi.org/10.1038/s41586-022-05655-4
Yamanaka Y, Hamidi S, Yoshioka-Kobayashi K, Munira S, Sunadome K, Zhang Y, Kurokawa Y, Ericsson R, Mieda A, Thompson JL, Kerwin J, Lisgo S, Yamamoto T, Moris N, Martinez-Arias A, Tsujimura T, Alev C (2022) Reconstituting human somitogenesis in vitro. Nature. https://doi.org/10.1038/s41586-022-05649-2
Yaman YI, Ramanathan S (2023) Controlling human organoid symmetry breaking reveals signaling gradients drive segmentation clock waves. Cell 186:513–527.e19
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Miao, Y., Diaz-Cuadros, M., Pourquié, O. (2023). Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2023_507
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DOI: https://doi.org/10.1007/7651_2023_507
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3686-2
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