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Human Pre-gastrulation Embryo Culture in 3D Condition

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Embryo Models In Vitro

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2767))

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Abstract

The development process of human embryo until blastocyst is well understood during the past 30 years, however, embryogenesis from blastocyst to pre-gastrulation was still remained a “black box”. Limited by research materials and culture technologies, the “black box” is still unopened. We recently established an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537–542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (82160294), the Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University (202101AY070001-260), and Open Project of Yunnan Provincial Reproductive and Obstetrics and Gynecology Clinical Medicine Center (zx2019-01-01and 2020LCZXKF-SZ04).

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Authors and Affiliations

  1. Department of Reproductive Medicine, The First People’s Hospital of Yunnan Province, Kunming, China

    Lifeng Xiang

  2. State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China

    Lifeng Xiang, Yu Yin, Gaohui Shi & Tianqing Li

Authors
  1. Lifeng Xiang
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  2. Yu Yin
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  3. Gaohui Shi
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  4. Tianqing Li
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Editor information

Editors and Affiliations

  1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA

    Magdalena Zernicka-Goetz

  2. Ottawa, ON, Canada

    Kursad Turksen

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Xiang, L., Yin, Y., Shi, G., Li, T. (2023). Human Pre-gastrulation Embryo Culture in 3D Condition. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2022_466

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  • DOI: https://doi.org/10.1007/7651_2022_466

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3685-5

  • Online ISBN: 978-1-0716-3686-2

  • eBook Packages: Springer Protocols

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