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Imaging Mass Spectrometry

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Laura M Cole

    1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Malcolm R Clench

    1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2688)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. MALDI and Trace Metal Analysis in Age-Related Macular Degeneration

    • Joshua Millar, Susan Campbell, Catherine Duckett, Sarah Doyle, Laura M. Cole
    Pages 1-13

  3. HistoSnap: A Novel Software Tool to Extract m/z-Specific Images from Large MSHC Datasets

    • Kenneth Verheggen, Nivedita Bhattacharya, Marthe Verhaert, Bram Goossens, Raf Sciot, Peter Verhaert
    Pages 15-26

  4. Spatially Resolved Quantitation of Drug in Skin Equivalents Using Mass Spectrometry Imaging (MSI)

    • Cristina Russo, Malcolm R. Clench
    Pages 27-40

  5. Update DESI Mass Spectrometry Imaging (MSI)

    • Emmanuelle Claude, Mark Towers, Emrys Jones
    Pages 41-54

  6. Liquid Extraction Surface Analysis Mass Spectrometry Imaging of Denatured Intact Proteins

    • Emma K. Sisley, James W. Hughes, Oliver J. Hale, Helen J. Cooper
    Pages 55-62

  7. MALDI MS Imaging of Cucumbers

    • Robert Bradshaw
    Pages 63-69

  8. The Adaptation of the QV600 LLI Milli-Fluidics System to House Ex Vivo Gastrointestinal Tissue Suitable for Drug Absorption and Permeation Studies, Utilizing MALDI MSI and LC-MS/MS

    • Chloe E. Spencer, Catherine J. Duckett, Stephen Rumbelow, Malcolm R. Clench
    Pages 71-82

  9. Ambient Mass Spectrometry Imaging by Water-Assisted Laser Desorption/Ionization for Ex Vivo and in Vivo Applications

    • Nina Ogrinc, Paul Chaillou, Alexandre Kruszewski, Cristian Duriez, Michel Salzet, Isabelle Fournier
    Pages 83-94

  10. Cytological Cytospin Preparation for the Spatial Proteomics Analysis of Thyroid Nodules Using MALDI-MSI

    • Isabella Piga, Fabio Pagni, Fulvio Magni, Andrew Smith
    Pages 95-105

  11. Matrix Effects Free Imaging of Thin Tissue Sections Using Pneumatically Assisted Nano-DESI MSI

    • Leonidas Mavroudakis, Ingela Lanekoff
    Pages 107-121

  12. Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials

    • Joseph Ready, Callie Seaman
    Pages 123-133

  13. Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging

    • Maria K. Andersen, Marco Giampà, Elise Midtbust, Therese S. Høiem, Sebastian Krossa, May-Britt Tessem
    Pages 135-146

  14. Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model

    • Lucy Flint
    Pages 147-159

  15. Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue

    • Marco Giampà, Maria K. Andersen, Sebastian Krossa, Vanna Denti, Andrew Smith, Siver Andreas Moestue
    Pages 161-172

  16. Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS

    • Jens Soltwisch, Bram Heijs
    Pages 173-186

  17. MS1-Based Data Analysis Approaches for FFPE Tissue Imaging of Endogenous Peptide Ions by Mass Spectrometry Histochemistry (MSHC)

    • Nivedita Bhattacharya, Konstantin Nagornov, Kenneth Verheggen, Marthe Verhaert, Raf Sciot, Peter Verhaert
    Pages 187-202

  18. Perspective: Mass Spectrometry Imaging – The Next 5 Years

    • Malcolm R. Clench, Laura M. Cole
    Pages 203-210

  19. Back Matter

    Pages 211-212
    Download chapter PDF
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Keywords

About this book

This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchersand experts looking to expand their knowledge. 

Editors and Affiliations

  • Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Laura M Cole, Malcolm R Clench

Bibliographic Information

  • Book Title: Imaging Mass Spectrometry

  • Book Subtitle: Methods and Protocols

  • Editors: Laura M Cole, Malcolm R Clench

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3319-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3318-2Published: 07 July 2023

  • Softcover ISBN: 978-1-0716-3321-2Due: 07 August 2023

  • eBook ISBN: 978-1-0716-3319-9Published: 06 July 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 212

  • Number of Illustrations: 5 b/w illustrations, 68 illustrations in colour

  • Topics: Mass Spectrometry, Materials Science, general

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