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Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue

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Imaging Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2688))

Abstract

Molecular visualization of metabolites, lipids, and proteins by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is becoming an in-demand analytical approach to aid the histopathological analysis of breast cancer. Particularly, proteins seem to play a role in cancer progression, and specific proteins are currently used in the clinic for staging. Formalin-fixed paraffin-embedded (FFPE) tissues are ideal for correlating the molecular markers with clinical outcomes due to their long-term storage. So far, to obtain proteomic information by MSI from this kind of tissue, antigen retrieval and tryptic digestion steps are required. In this chapter, we present a protocol to spatially detect small proteins in tumor and necrotic regions of patient-derived breast cancer xenograft FFPE tissues without employing any on-tissue digestion. This protocol can be used for other kinds of FFPE tissue following specific optimization of the sample preparation phases.

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Author information

Authors and Affiliations

  1. Department of Clinical and Molecular Medicine, NTNU – Norwegian University of Science and Technology, Trondheim, Norway

    Marco Giampà & Siver Andreas Moestue

  2. Department of Circulation and Medical Imaging, NTNU – Norwegian University of Science and Technology, Trondheim, Norway

    Maria K. Andersen & Sebastian Krossa

  3. Proteomics and Metabolomics Unit, Department of Medicine and Surgery, Vedano al Lambro, Italy

    Vanna Denti & Andrew Smith

  4. Department of Pharmacy, Nord University, Bodø, Norway

    Siver Andreas Moestue

Authors
  1. Marco Giampà
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  2. Maria K. Andersen
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  3. Sebastian Krossa
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  4. Vanna Denti
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  5. Andrew Smith
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  6. Siver Andreas Moestue
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Corresponding author

Correspondence to Marco Giampà .

Editor information

Editors and Affiliations

  1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Laura M Cole

  2. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Malcolm R Clench

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Giampà, M., Andersen, M.K., Krossa, S., Denti, V., Smith, A., Moestue, S.A. (2023). Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3319-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3318-2

  • Online ISBN: 978-1-0716-3319-9

  • eBook Packages: Springer Protocols

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