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Spatially Resolved Quantitation of Drug in Skin Equivalents Using Mass Spectrometry Imaging (MSI)

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Imaging Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2688))

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has seen a growing interest as a leading technique in the pharmaceutical industry for mapping label-free exogenous and endogenous species in biological tissues. However, the use of MALDI-MSI to perform spatially resolved absolute quantitation of species directly in tissues is still challenging, and robust quantitative mass spectrometry imaging (QMSI) methods need to be developed. In this study, we describe the microspotting technique for analytical and internal standard deposition, matrix sublimation, powerful QMSI software, and mass spectrometry imaging setup to obtain absolute quantitation of drug distribution in 3D skin models.

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Acknowledgments

This work was supported by the Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre of Sheffield Hallam University, and Croda Plc. Innovenn (UK) Ltd.

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Authors and Affiliations

  1. Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Cristina Russo & Malcolm R. Clench

  2. Department of Natural Sciences, Middlesex University, London, UK

    Cristina Russo

Authors
  1. Cristina Russo
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  2. Malcolm R. Clench
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Corresponding author

Correspondence to Cristina Russo .

Editor information

Editors and Affiliations

  1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Laura M Cole

  2. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Malcolm R Clench

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Russo, C., Clench, M.R. (2023). Spatially Resolved Quantitation of Drug in Skin Equivalents Using Mass Spectrometry Imaging (MSI). In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3319-9_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3318-2

  • Online ISBN: 978-1-0716-3319-9

  • eBook Packages: Springer Protocols

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