Summary
In order to achieve even more widespread adoption over the next 5 years, a number of issues in mass spectrometry imaging need to be addressed. These are non-observation of compounds (due to ionization suppression), sample throughput, imaging of low-abundant species, and how to extract information from the large volumes of data generated. In this article, how current research indicates that these issues will be resolved along with potential application areas that MSI could look to exploit is discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Taylor AJ, Dexter A, Bunch J (2018) Exploring ion suppression in mass spectrometry imaging of a heterogeneous tissue. Anal Chem 90:5637
Challen B, Cramer R (2022) Advances in ionisation techniques for mass spectrometry-based omics research. Proteomics 22:2100394
Soltwisch J, Kettling H, Vens-Cappell S, Wiegelmann M, Muthing J, Dreisewerd K (2015) Mass spectrometry imaging with laser-induced postionization. Science 348:211–215
Steven RT, Shaw M, Dexter A, Murta T, Green FM, Robinson KN, Gilmore IS, Takats Z, Bunch J (2019) Construction and testing of an atmospheric-pressure transmission-mode matrix assisted laser desorption ionisation mass spectrometry imaging ion source with plasma ionisation enhancement. Anal Chim Acta 1051:110–119
Soltwisch J, Heijs B, Koch A, Vens-Cappell S, Höhndorf J, Dreisewerd K (2020) MALDI-2 on a trapped ion mobility quadrupole time-of-flight instrument for rapid mass spectrometry imaging and ion mobility separation of complex lipid profiles. Anal Chem 92:8697–8703
Trim PJ, Djidja M-C, Atkinson SJ, Oakes K, Cole LM, Anderson DMG, Hart PJ, Francese S, Clench MR (2010) Introduction of a 20 kHz Nd:YVO4 laser into a hybrid quadrupole time-of-flight mass spectrometer for MALDI-MS imaging. Anal Bioanal Chem 397:3409–3419
Simmons DA (2008) Improved MALDI-MS imaging performance using continuous laser rastering. Appl Biosyst Tech Note 1–5
McDonnell LA, Heeren RMA (2007) Imaging mass spectrometry. Mass Spectrom Rev 26:606–643
Guo A, Burleigh RJ, Smith N, Brouard M, Burt M (2020) High-resolution ion microscope imaging over wide mass ranges using electrodynamic postextraction differential acceleration. J Am Soc Mass Spectrom 31:1903
Körber A, Keelor JD, Claes BSR, Heeren RMA, Anthony IGM (2022) Fast mass microscopy: mass spectrometry imaging of a gigapixel image in 34 minutes. Anal Chem (Washington) 94:14652
Staab D, Morandi G, Stoeckli M (2013) Mass spectrometric imaging applied to biomedical research. Chimia 67:296
Henderson F, Jones E, Denbigh J, Christie L, Chapman R, Hoyes E, Claude E, Williams KJ, Roncaroli F, McMahon A (2020) 3D DESI-MS lipid imaging in a xenograft model of glioblastoma: a proof of principle. Sci Rep 10:16512
Caprioli RM, Farmer TB, Gile J (1997) Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. Anal Chem 69:4751–4760
Giesen C, Wang HAO, Schapiro D, Zivanovic N, Jacobs A, Hattendorf B, Schüffler PJ, Grolimund D, Buhmann JM, Brandt S, Varga Z, Wild PJ, Günther D, Bodenmiller B (2014) Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nat Methods 11:417–422
Le Rochais M, Hemon P, Pers J-O, Uguen A (2022) Application of high-throughput imaging mass cytometry hyperion in cancer research. Front Immunol 13:859414
Yagnik G, Liu Z, Rothschild KJ, Lim MJ (2021) Highly multiplexed immunohistochemical MALDI-MS imaging of biomarkers in tissues. J Am Soc Mass Spectrom 32:977–988
Corr JJ, Kovarik P, Schneider BB, Hendrikse J, Loboda A, Covey TR (2006) Design considerations for high speed quantitative mass spectrometry with MALDI ionization. J Am Soc Mass Spectrom 17:1129–1141
Lamont L, Eijkel GB, Jones EA, Flinders B, Ellis SR, Porta Siegel T, Heeren RMA, Vreeken RJ (2018) Targeted drug and metabolite imaging: desorption electrospray ionization combined with triple quadrupole mass spectrometry. Anal Chem 90:13229–13235
Lamont L, Hadavi D, Viehmann B, Flinders B, Heeren RMA, Vreeken RJ, Porta Siegel T (2021) Quantitative mass spectrometry imaging of drugs and metabolites: a multiplatform comparison. Anal Bioanal Chem 413:2779–2791
Hale OJ, Cooper HJ (2021) Native mass spectrometry imaging of proteins and protein complexes by nano-DESI. Anal Chem 93:4619
Illes-Toth E, Ramos MR, Cappai R, Dalton C, Smith DP (2015) Distinct higher-order α-synuclein oligomers induce intracellular aggregation. Biochem J 468:485–493
Li H, Amanda B (2011) Hummon imaging mass spectrometry of three-dimensional cell culture systems. Anal Chem 83:8794
Avery JL, McEwen A, Flinders B, Francese S, Clench MR (2011) Matrix-assisted laser desorption mass spectrometry imaging for the examination of imipramine absorption by Straticell-RHE-EPI/001 an artificial model of the human epidermis. Xenobiotica 41:735–742
Spencer CE, Flint LE, Duckett CJ, Cole LM, Cross N, Smith DP, Clench MR (2020) Role of MALDI-MSI in combination with 3D tissue models for early stage efficacy and safety testing of drugs and toxicants. Expert Rev Proteomics 17:827–841
Hu H, Laskin J (2022) Emerging computational methods in mass spectrometry imaging. Adv Sci 9:e2203339–e2203n/a
Zhang Q, Burrage MK, Lukaschuk E, Shanmuganathan M, Popescu IA, Nikolaidou C, Mills R, Werys K, Hann E, Barutcu A, Polat SD, null n, Salerno M, Jerosch-Herold M, Kwong RY, Watkins HC, Kramer CM, Neubauer S, Ferreira VM, Piechnik SK (2021) Toward replacing late gadolinium enhancement with artificial intelligence virtual native enhancement for gadolinium-free cardiovascular magnetic resonance tissue characterization in hypertrophic cardiomyopathy. Circulation 144:589–599
Abdelmoula WM, Lopez BG, Randall EC, Kapur T, Sarkaria JN, White FM, Agar JN, Wells WM, Agar NYR (2021) Peak learning of mass spectrometry imaging data using artificial neural networks. Nat Commun 12:5544
Borodinov N, Lorenz M, King ST, Ievlev AV, Ovchinnikova OS (2020) Toward nanoscale molecular mass spectrometry imaging via physically constrained machine learning on co-registered multimodal data. NPJ Computat Mater 6:83
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Clench, M.R., Cole, L.M. (2023). Perspective: Mass Spectrometry Imaging – The Next 5 Years. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_17
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3319-9_17
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3318-2
Online ISBN: 978-1-0716-3319-9
eBook Packages: Springer Protocols