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Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model

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Imaging Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2688))

Abstract

Multimodal mass spectrometry imaging (MSI) is a leading approach for investigating the molecular processes within biological samples. The parallel detection of compounds including metabolites, lipids, proteins, and metal isotopes allows for a more holistic understanding of tissue microenvironments. Universal sample preparation is a primary enabler for samples of the same set to be run across multiple modalities. Using the same method and materials for a cohort of samples reduces any potential variability during sample preparation and allows for comparable analysis across analytical imaging techniques. Here, the MSI workflow is describing a sample preparation protocol for the analysis of three-dimensional (3D) cell culture models. The analysis of biologically relevant cultures by multimodal MSI offers a method in which models of cancer and disease can be studied for the use in early-stage drug development.

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Author information

Authors and Affiliations

  1. Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Sheffield, UK

    Lucy Flint

  2. Imaging and Data Analytics, Clinical Pharmacology and Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK

    Lucy Flint

Authors
  1. Lucy Flint
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Corresponding author

Correspondence to Lucy Flint .

Editor information

Editors and Affiliations

  1. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Laura M Cole

  2. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

    Malcolm R Clench

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Flint, L. (2023). Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3319-9_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3318-2

  • Online ISBN: 978-1-0716-3319-9

  • eBook Packages: Springer Protocols

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