Abstract
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a sensitive technique which enables fast, spatially resolved analysis of elements at trace concentration levels in a range of solid sample types, including plant materials. Within this chapter, we describe how to prepare leaf material and seeds for elemental distribution imaging, how to embed material in gelatin and epoxy resin, how to produce matrix-matched reference materials, and how to optimize laser ablation methods.
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Acknowledgments
Many thanks to Dr. Rob Hutchinson from Elemental Scientific Lasers, for the applications support with the laser ablation unit and for his advice with the bioimaging optimization section.
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Ready, J., Seaman, C. (2023). Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials. In: Cole, L.M., Clench, M.R. (eds) Imaging Mass Spectrometry. Methods in Molecular Biology, vol 2688. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3319-9_11
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DOI: https://doi.org/10.1007/978-1-0716-3319-9_11
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Publisher Name: Humana, New York, NY
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