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Plant Metabolic Engineering

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Vladimir Shulaev

    1. Department of Biological Sciences, University of North Texas, Denton, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2396)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Methods for the Development of Recombinant Microorganisms for the Production of Natural Products

    • Alexander Perl, Hunter Dalton, YeJong Yoo, Mattheos A. G. Koffas
    Pages 1-17

  3. Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus Byproducts

    • Salvatore Multari, Fulvio Mattivi, Stefan Martens
    Pages 19-27

  4. Reconstitution of Metabolic Pathway in Nicotiana benthamiana

    • Chenggang Liu
    Pages 29-33

  5. A Protocol for Phylogenetic Reconstruction

    • Soham Sengupta, Rajeev K. Azad
    Pages 35-46

  6. RNA-Seq Data Analysis Pipeline for Plants: Transcriptome Assembly, Alignment, and Differential Expression Analysis

    • David J. Burks, Rajeev K. Azad
    Pages 47-60

  7. A Protocol for Horizontally Acquired Metabolic Gene Detection in Algae

    • Ravi S. Pandey, Rajeev K. Azad
    Pages 61-69

  8. Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction Cartridges

    • Khadiza Zaman, Prajita Pandey, Vladimir Shulaev, Laszlo Prokai
    Pages 71-84

  9. Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization High-Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L) Berry

    • Johana S. Revel, Armando Alcázar Magaña, Jeffrey Morré, Laurent Deluc, Claudia S. Maier
    Pages 85-99

  10. GC-MS/MS Profiling of Plant Metabolites

    • Feroza Kaneez Choudhury, Prajita Pandey, Ron Meitei, Dwain Cardona, Amit C. Gujar, Vladimir Shulaev
    Pages 101-115

  11. Analysis of Grape Volatiles Using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based Metabolomics

    • Manoj Ghaste, Fulvio Mattivi, Giuseppe Astarita, Vladimir Shulaev
    Pages 117-136

  12. A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites

    • Giuseppe Paglia, Giuseppe Astarita
    Pages 137-159

  13. Macrolipidomic Profiling of Vegetable Oils: The Analysis of Sunflower Oils with Different Oleic Acid Content

    • Juan J. Aristizabal-Henao, Ken D. Stark
    Pages 161-173

  14. Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry

    • Giorgis Isaac, Vladimir Shulaev, Robert S. Plumb
    Pages 175-186

  15. Comprehensive Analysis of Plant Lipids Using Sub-2-μm Particle CO2-Based Chromatography Coupled to Mass Spectrometry

    • Carolina Salazar, Michael D. Jones, Giorgis Isaac, Vladimir Shulaev
    Pages 187-195

  16. Bioinformatics in Lipidomics: Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid Software

    • Ningombam Sanjib Meitei, Vladimir Shulaev
    Pages 197-214

  17. A Protocol for Prion Discovery in Plants

    • Jamie D. Dixson, Rajeev K. Azad
    Pages 215-226

  18. Structural Determination of Uridine Diphosphate Glycosyltransferases Using X-Ray Crystallography

    • Kasandra Alderete, Xiaoqiang Wang
    Pages 227-241

  19. Back Matter

    Pages 243-244
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Keywords

About this book

This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering. 


Editors and Affiliations

  • Department of Biological Sciences, University of North Texas, Denton, USA

    Vladimir Shulaev

Bibliographic Information

  • Book Title: Plant Metabolic Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Vladimir Shulaev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1822-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1821-9Published: 17 November 2021

  • Softcover ISBN: 978-1-0716-1824-0Published: 18 November 2022

  • eBook ISBN: 978-1-0716-1822-6Published: 16 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 244

  • Number of Illustrations: 14 b/w illustrations, 55 illustrations in colour

  • Topics: Plant Sciences, Bioinformatics

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