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Structural Determination of Uridine Diphosphate Glycosyltransferases Using X-Ray Crystallography

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Plant Metabolic Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2396))

Abstract

Uridine diphosphate glycosyltransferases (UGTs) are the key enzymes in glycosylation processes for decorating plant natural products with sugars. Crystallography, one of the powerful techniques for determining protein structures, was used as the main experimental technique and combined with biochemical methods to study the structure–function relationship and molecular mechanisms of UGTs. Crystal structures of plant UGTs have revealed their exquisite architectures and provided the structural basis for understanding their catalytic mechanism and substrate specificity. In this chapter, some protocols and experimental details of all key stages of protein structure determination are provided, and the structural insights on plant UGTs are also highlighted in combination of method description.

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Authors and Affiliations

  1. Department of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA

    Kasandra Alderete & Xiaoqiang Wang

Authors
  1. Kasandra Alderete
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  2. Xiaoqiang Wang
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Corresponding author

Correspondence to Xiaoqiang Wang .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of North Texas, Denton, TX, USA

    Vladimir Shulaev

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Alderete, K., Wang, X. (2022). Structural Determination of Uridine Diphosphate Glycosyltransferases Using X-Ray Crystallography. In: Shulaev, V. (eds) Plant Metabolic Engineering. Methods in Molecular Biology, vol 2396. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1822-6_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1822-6_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1821-9

  • Online ISBN: 978-1-0716-1822-6

  • eBook Packages: Springer Protocols

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