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Structural Proteomics

High-Throughput Methods

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Raymond J. Owens

    1. The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2305)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Structural Bioinformatics

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Protein Data Bank Archive

      • Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffrey C. Hoch, John L. Markley
      Pages 3-21

    3. Computational Methods for the Elucidation of Protein Structure and Interactions

      • Nicholas S. Edmunds, Liam J. McGuffin
      Pages 23-52

    4. Methods for Molecular Modelling of Protein Complexes

      • Tejashree Rajaram Kanitkar, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha et al.
      Pages 53-80

  3. Protein Production

    1. Front Matter

      Pages 81-81
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    2. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus

      • Ester Behiels, Jonathan Elegheert
      Pages 83-104

    3. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small Scale: Application for Structural Studies

      • Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley et al.
      Pages 105-128

    4. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

      • Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, Joop van den Heuvel
      Pages 129-140

    5. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering

      • Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, Imre Berger
      Pages 141-152

    6. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification

      • Sylvain Geny, Simon Pichard, Arnaud Poterszman, Jean-Paul Concordet
      Pages 153-174

    7. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems

      • Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, Stefan Kubick
      Pages 175-190Open Access

  4. Structure Determination

    1. Front Matter

      Pages 191-191
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    2. Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

      • Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, A. S. Jijumon et al.
      Pages 193-201

    3. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources

      • Pierre Aller, Allen M. Orville
      Pages 203-228

    4. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example

      • Christoph A. Diebolder, Rebecca S. Dillard, Ludovic Renault
      Pages 229-256

    5. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

      • Carlos Oscar S. Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo et al.
      Pages 257-289

    6. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection

      • Ottilie von Loeffelholz, Bruno P. Klaholz
      Pages 291-299

    7. Cryo-Focused Ion Beam Lamella Preparation Protocol for in Situ Structural Biology

      • Jana Moravcová, Radka Dopitová, Matyáš Pinkas, Jiří Nováček
      Pages 301-322

    8. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED

      • Emma Danelius, Tamir Gonen
      Pages 323-342
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Keywords

About this book

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.


Chapter 9 is available open access under a CC BY 4.0 license.

Editors and Affiliations

  • The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK

    Raymond J. Owens

Bibliographic Information

  • Book Title: Structural Proteomics

  • Book Subtitle: High-Throughput Methods

  • Editors: Raymond J. Owens

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1406-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1405-1Published: 06 May 2021

  • Softcover ISBN: 978-1-0716-1408-2Published: 07 May 2022

  • eBook ISBN: 978-1-0716-1406-8Published: 05 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 344

  • Number of Illustrations: 21 b/w illustrations, 67 illustrations in colour

  • Topics: Life Sciences, general, Chemistry/Food Science, general

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