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Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

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Structural Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2305))

Abstract

The expression of mammalian recombinant proteins in insect cell lines using transient-plasmid-based gene expression enables the production of high-quality protein samples. Here, the procedure for virus-free transient gene expression (TGE) in High Five insect cells is described in detail. The parameters that determine the efficiency and reproducibility of the method are presented in a robust protocol for easy implementation and set-up of the method. The applicability of the TGE method in High Five cells for proteomic, structural, and functional analysis of the expressed proteins is shown.

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Authors and Affiliations

  1. Department of Biotechnology, Institut fuer Biochemie, Biotechnologie und Bioinformatik, Technische Universitaet Braunschweig, Braunschweig, Germany

    Maren Schubert & Federico Bertoglio

  2. RG Cellular Proteomics, Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany

    Manfred Nimtz

  3. Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany

    Stefan Schmelz, Peer Lukat & Joop van den Heuvel

Authors
  1. Maren Schubert
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  2. Manfred Nimtz
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  3. Federico Bertoglio
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  4. Stefan Schmelz
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  5. Peer Lukat
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  6. Joop van den Heuvel
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Corresponding author

Correspondence to Joop van den Heuvel .

Editor information

Editors and Affiliations

  1. The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK

    Raymond J. Owens

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Schubert, M., Nimtz, M., Bertoglio, F., Schmelz, S., Lukat, P., van den Heuvel, J. (2021). Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells. In: Owens, R.J. (eds) Structural Proteomics. Methods in Molecular Biology, vol 2305. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1406-8_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1406-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1405-1

  • Online ISBN: 978-1-0716-1406-8

  • eBook Packages: Springer Protocols

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