Abstract
Grain protein content (GPC) in durum wheat is a crucial determinant of pasta quality and as such is an important economic factor. This study was carried out to determine the microsatellite markers (SSRs) as associated with GPC in durum wheat grown under normal and moisture stress conditions. F3 and F4 population derived from 151 F2 individuals developed from a cross between Oste-Gata (drought tolerant) and Massara-1 (drought susceptible) genotypes, were used. The population was evaluated under four environmental conditions (two irrigation regimes in two growing seasons). The results of single marker regression analysis (SMA) revealed that 2, 4 and 10 markers to be associated with GPC, test weight (TW) and 1000 grain weight (TGW), respectively. These markers explained between 4.4 and 21.8% of the phenotypic variation in either environmental condition. The most significant marker observed for GPC was located on 5B chromosome near Xgwm408 under normal conditions and the other marker was observed on 1A, explaining about 15% of phenotypic variance. However, it was not recognized any marker related to GPC under drought stress conditions. Xgwm408 marker was coincident with the markers identified for TW, TGW and components of grain yield under drought stress conditions. In spite of 5B, the other chromosomes such as 2B and 3B were related to quantitative traits like TW and TGW. Composite interval mapping (CIM) identified 4 and 5 putative minor and major QTL for TW and TGW, respectively. Two QTL near Xbarc101 and Xbarc124 markers on 3B and 2B chromosome, explained up to 45.2 and 6% of phenotypic variations of TGW and TW, respectively.
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Abdalla, O., Dieseth, J.A., Singh R.P. 1992. Breeding durum wheat at CIMMYT. In: Rajaram, S., Saari, E.E., Hettel, G.P. (eds), Durum Wheats: Challenges and Opportunities. CIMMYT, Mexico, pp. 1–13.
Arzani, A. 2002. Grain yield performance of durum wheat germplasm under Iranian dryland and irrigated field conditions. Sabrao J. Breed. Genet. 34:9–18.
Blanco, A., de Giovanni, C., Laddomada, B., Sciancalepore, A., Simeone, R., Devos, K.M., Gale, M.D. 1996. Quantitative trait loci influencing grain protein content in tetraploid wheats. Plant Breed. 115:310–316.
Blanco, A., Simeone, R., Gadaleta, A. 2006. Detection of QTLs for grain protein content in durum wheat. Theor. Appl. Genet. 112:1195–1204.
Basten, C.J., Weir, B.S., Zeng, Z.B. 1994. Z map-a QTL Cartographer. In: Smith, C., Gavora, J.S., Benkel, J., Chesnias, B., Fiarfull, W., Gibson, J.P., Kennedy, B.W., Burnsid, E.B. (eds), Proc. 5th World Congress on Genetic Applied to Livestock Production: Computing Strategies and Software. Guelph, Ontario, Canada.
Bathia, C.R., Rabson, R. 1987. Relationship of grain yield and nutritional quality. In: Nutritional Quality of Cereal Grains: Genetic and Agronomic Improvement. Agronomy Monograph no. 28, ASA-CSSA-SSSA, Madison, WI, USA, pp. 11–43.
CWC 2005. Description of durum wheat semolina quality factors. Available online at: https://doi.org/www.californiawheat.org
Dellaporta, S.L., Wood, J., Hinks, J.B. 1983. A plant DNA minipreparation: Ver. II, Mol. Biol. Plant Rep. 1:19–21.
Dexter, J.E., Marchylo, B.A. 2000. Recent trends in durum wheat and pasta processing. Impact on Durum wheat Quality Requirements. In: Durum Wheat, Semolina and Pasta Quality. Recent Achievements and New Trends. Proc. of the International Workshop, Montpellier 27 November 2000, Institute National of Research Agronomy, Montpellier, France, pp. 77–101.
De Vita, P., Riefolo, C., Codianni, P., Cattivelli, L., Fares, C. 2006. Agronomic and qualitative traits of T. turgidum ssp. Dicoccum genotypes cultivated in Italy. Euphytica 150:195–205.
Dholakia, B.B., Ammiraju, J.S.S., Santra, D.K., Singh, H., Katti, M.V., Lagu, M.D., Tamhankar, S.A., Rao, V.S., Gupta, V.S., Dhaliwal, H.S. et al. 2001. Molecular marker analysis of protein content using PCR-based markers in wheat. Biochemical Genet. 39:325–338.
Elias, E.M., Steiger, D.K., Cantrell, R.G. 1996. Evaluation of lines derived from wild emmer chromosome substitutions. II. Agronomic traits. Crop Sci. 36:228–233.
Elouafi, I., Nachit, M.M. 2004. A genetic linkage map of the Durum × Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theor. Appl. Genet. 108:401–413.
Fulton, T.M., Grandillo, S., Beck-Bunn, T., Fridman, E., Frampton, A., Lopez, J., Petiard, V., Uhlig, J., Zamir, D., Tanksley, S.D. 2000. Advanced backcross QTL analysis of a Lycopersicon esculentum × Lycopersicon parviflorum cross. Theor. Appl. Genet. 100:1025–1042.
Giura, 2003. Genetic effect of chromosome 7B on some quality traits and earliness in wheat. In: Nistor, G. (ed.), Biotechnologie si Biodiversitate. USAMVB-Timisoara, Ed. Agroprint, Timisoar, Romania, pp. 271–277.
Giura, A., Contescu, L., Mustatea, P., Ittu, G., Saulescu, N.N. 2008. Effects of chromosome 7B genes on grain protein concentration, yield and earliness in wheat. Cereal Res. Commun. 36:669–676.
Golabadi, M., Arzani, A., Mirmohammadi Maibody, S.A.M., Sayed Tabatabaei, B.E., Mohammadi, S.A. 2011. Identification of microsatellite markers linked with yield components under drought stress at terminal growth stages in durum wheat. Euphytica 177:207–221.
Groos, C., Robert, N., Bervas, E., Charmet, G. 2003. Genetic analysis of grain protein-content, grain yield and thousand-kernel weight in bread wheat Theor. Appl. Genet. 106:1032–1040.
Huang, X.Q., Cloutier, S., Lycar, L., Radovanovic, N., Humphreys, D.G., Noll, J.S., Somers, D.J., Brown, P.D. 2006. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheat (Triticum aestivum L.). Theor. Appl. Genet. 113:753–766.
Hernandez, G.J.L., Elias, E.M., Kianian, S.F. 2004. Mapping genes for grain protein concentration and grain yield on chromosome 5B of Triticum turgidum (L.) var. dicoccoides. Euphytica 139:217–225.
Jolly, C.J., Glenn, G.M., Rahman, S. 1996. GSP-1 genes are linked to the grain hardiness locus (Ha) on wheat chromosome 5D. Proc. Natl. Acad. Sci. USA 93:2408–2413.
Joppa, L.R., Cantrell, R.G. 1990. Chromosomal location of genes for grain protein content of wild tetraploid wheat. Crop Sci. 30:1059–1064.
Liu, C., Gale, M.D. 1989. Ibf-1 (iodine binding factor), a high variable marker system in the Triticeae. Theor. Appl. Genet. 77:233–240.
Matsuo, R.R., Dexter, J.E. 1980. Relationship between durum wheat physical characteristics and semolina milling properties properties. Can. J. Plant Sci. 60:49–53.
Manly, K.F., Olson, J.M. 1999. Overview of QTL mapping software and introduction to map manager QT. Mamm. Genome 10:327–334.
Narasimhamoorthy, B., Gill, B.S., Fritz, A.K., Nelson, J.C., Brown-Guedira, G.L. 2006. Advanced backcross QTL analysis of a hard winter wheat x synthetic wheat population. Theor. Appl. Genet. 112:787–796.
Perretant, M.R., Cadalen, T., Charmet, G., Sourdille, P., Nicolas, P., Boeuf, C., Tixier, M.H., Branlard, G., Bernard, S. 2000. QTL analysis of bread-making quality in wheat using a doubled haploid population. Theor. Appl. Genet. 100:1167–1175.
Petrova, I. 2007. End-use quality of Bulgarian durum wheat. Bulg. J. Agric. Sci. 13:161–169.
Prasad, M., Kumar, N., Kulwal, P.L., Roder, M.S., Balyan, H.S., Dhaliwal, H.S., Gupta, P.K. 2003. QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor. Appl. Genet. 106:659–667.
Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.
SAS Institute 2000. The SAS System for Windows. Release 8.01, SAS Inst. Inc., Cary, NC, USA.
Simmonds, N.W. 1995. The relation between yield and protein in cereal grain. J. Sci. Food Agric. 67:309–315.
Somers, D.J., Isaac, P., Edwards, K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.
Suprayogi, Y., Pozniak, C.J., Clarke, F.R., Clarke, J.M., Knox, R.E., Singh, A.K. 2009. Identification and validation of quantitative trait loci for grain protein concentration in adapted Canadian durum wheat populations. Theor. Appl. Genet. 119:438–448.
Steiger, D.K., Elias, E.M., Cantrell, R.G. 1996. Evaluation of lines derived from wild emmer chromosome substitutions. I. Quality traits. Crop Sci. 36:223–227.
Tanksley, S.D., Grandillo, S., Fulton, T.M., Zamir, D., Eshed, Y., Petiard, V., Lopez, J., BeckBunn, T. 1996. Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L-pimpinellifolium.Theor. Appl. Genet. 92:213–224.
Villareal, R.L., Banuelos, O., Mujeed-Razi, A. 1997. Agronomic performance of related durum wheat (Triticum turgidum L.) stocks possessing the chromosome substitution T1BL.1RS. Crop Sci. 37:1735–1740.
Wang, S., Basten, C.J., Zeng, Z. 2004. Windows QTL cartographer. V2.0 Program in statistical genetics, North Carolina State University, North Carolina, USA. Available online via DIALOG at: https://doi.org/www.statgen.ncsu.edu/qtlcart/WQTLCart.htm
Werner-Fraczek, J.E., Close, T.J. 1998. Genetic studies of Triticeae dehydrins: Assignment of seed proteins and a regulatory factor to map positions. Theor. Appl. Genet. 97:220–226.
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Golabadi, M., Arzani, A. & Mirmohammadi Maibody, S.A.M. Identification of Microsatellite Markers Associated with Grain Protein Content in Durum Wheat Grown under Drought Stress at Terminal Growth Stages. CEREAL RESEARCH COMMUNICATIONS 40, 215–224 (2012). https://doi.org/10.1556/CRC.40.2012.2.6
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DOI: https://doi.org/10.1556/CRC.40.2012.2.6