Skip to main content
SpringerLink
Log in

Molecular genetic analysis of grain protein content and flour whiteness degree using RILs in common wheat

  • RESEARCH ARTICLE
  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

Grain protein content (GPC) and flour whiteness degree (FWD) are important qualitative traits in common wheat. Quantitative trait locus (QTL) mapping for GPC and FWD was conducted using a set of 131 recombinant-inbred lines derived from the cross ‘Chuan 35050 × Shannong 483’ in six environmental conditions. A total of 22 putative QTLs (nine GPC and 13 FWD) were identified on 12 chromosomes with individual QTL explaining 4.5–34.0% phenotypic variation. Nine QTLs (40.9%) were detected in two or more environments. The colocated QTLs were on chromosomes 1B and 4B. Among the QTLs identified for GPC, QGpc.sdau-4A from the parent Shannong 483 represented some important favourable QTL alleles. QGpc.sdau-2A.1 and QFwd.sdau-2A.1 had a significant association with both GPC and FWD. The markers detected on top of QTL regions could be potential targets for marker-assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2

Similar content being viewed by others

References

  • Charmet G., Robert N., Branlard G., Linossier L., Martre P. and Triboï E. 2005 Genetic analysis of dry matter and nitrogen accumulation and protein composition in wheat kernels. Theor. Appl. Genet. 111, 540–550.

    Article  CAS  PubMed  Google Scholar 

  • Distelfeld A., Uauy C., Olmos S., Schlatter A. R., Dubcovsky J. and Fahima T. 2004 Microcolinearity between a 2-cM region encompassing the grain protein content locus Gpc-6B1 on wheat chromosome 6 and a 350-kb region on rice chromosome 2. Funct. Integr. Genomic. 4, 59–66.

    Article  CAS  Google Scholar 

  • Distelfeld A., Uauy C., Fahima T. and Dubcovsky J. 2006 Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker. New Phytol. 169, 753–763.

    Article  CAS  PubMed  Google Scholar 

  • Fan Y. D., Sun H. Y., Zhao J. L., Ma Y. M., Li R. J. and Li S. S. 2009 QTL mapping for quality traits of northern-style hand-made Chinese steamed bread. J. Cereal Sci. 49, 225–229.

    Article  CAS  Google Scholar 

  • He Z. H., Yang J., Zhang Y., Quail K. J. and Peña R. J. 2004 Pan bread and dry white Chinese noodle quality in Chinese winter wheats. Euphytica 139, 257–267.

    Article  Google Scholar 

  • Joppa L. R., Du C., Hart G. E. and Hareland G. A. 1997 Mapping gene(s) for grain protein in tetraploid wheat (Triticum turgidum L.) using a population of recombinant inbred chromosome lines. Crop Sci. 37, 1586–1589.

    Article  CAS  Google Scholar 

  • Kuchel H., Langridge P., Mosionek L., Williams K. and Jefferies S. P. 2006 The genetic control of milling yield, dough rheology and baking quality of wheat. Theor. Appl. Genet. 112, 1487–1495.

    Article  CAS  PubMed  Google Scholar 

  • Li S. S., Jia J. Z., Wei X. Y., Zhang X. C., Chen H. M., Sun H. Y. et al. 2007 A intervarietal genetic map and QTL analysis for yield traits in wheat. Mol. Breed. 20, 167–178.

    Article  Google Scholar 

  • Liu J. J., He Z. H., Zhao Z. D., Peña R. J. and Rajaram S. 2003 Wheat quality traits and quality parameters of cooked dry white Chinese noodles. Euphytica 131, 147–154.

    Article  CAS  Google Scholar 

  • Liu W., Zhang Z. J. and Yang L. 2006 Chemiluminescence microfluidic chip fabricated in PMMA for determination of benzoyl peroxide in flour. Food Chem. 95, 693–698.

    Article  Google Scholar 

  • Mares D. J. and Campbell A. W. 2001 Mapping components of flour and noodle color in Australian wheat. Aust. J. Agric. Res. 52, 1297–1309.

    Article  CAS  Google Scholar 

  • McCartney C. A., Somers D. J., Lukow O., Ames N., Noll J., Cloutier S. et al. 2006 QTL analysis of quality traits in the spring wheat cross RL 4452 × ‘AC Domain’. Plant Breeding 125, 565–575.

    Article  CAS  Google Scholar 

  • Olmos S., Diestelfeld A., Chicaiza O., Schatter A. R., Fahima T., Echenique V. et al. 2003 Precise mapping of a locus affecting grain protein content in durum wheat. Theor. Appl. Genet. 107, 1243–1251.

    Article  CAS  PubMed  Google Scholar 

  • Patil R. M., Oak M. D., Tamhankar S. A., Sourdille P. and Rao V. S. 2008 Mapping and validation of a major QTL for yellow pigment content on 7AL in durum wheat (Triticum turgidum L. ssp. durum). Mol. Breed. 21, 485–496.

    Article  Google Scholar 

  • Payne P. I., Nightingale M. A., Krattiger A. F. and Holt L. M. 1987 The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties . J. Sci. Food Agr. 40, 51–65.

    Article  CAS  Google Scholar 

  • Pozniak C. J., Knox R. E., Clarke F. R. and Clarke J. M. 2007 Identification of QTL and association of a phytoene synthase gene with endosperm colour in durum wheat. Theor. Appl. Genet. 114, 525–537.

    Article  CAS  PubMed  Google Scholar 

  • Prasad M., Kumar N., Kulwal P. L., Röder M. S., Balyan H. S., Dhaliwal H. S. et al. 2003 QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor. Appl. Genet. 106, 659–667.

    CAS  PubMed  Google Scholar 

  • Radovanovic N., Cloutier S., Brown D., Humphreys D. G. and Lukow O. M. 2002 Genetic variance for gluten strength contributed by high molecular weight glutenin proteins. Cereal Chem. 79, 843–849.

    Article  CAS  Google Scholar 

  • Raman R., Raman H. and Martin P. 2007 Functional gene markers for polyphenol oxidase locus in bread wheat (Triticum aestivum L.). Mol. Breed. 19, 315–328.

    Article  CAS  Google Scholar 

  • Sun H. Y., Lü J. H., Fan Y. D., Zhao Y., Kong F. M., Li R. J. et al. 2008 Quantitative trait loci (QTLs) for quality traits related to protein and starch in wheat. Prog. Nat. Sci. 18, 825–831.

    Article  CAS  Google Scholar 

  • Sun X. Y., Wu K., Zhao Y., Kong F. M., Han G. Z., Jiang H. M. et al. 2009 QTL analysis of kernel shape and weight using recombinant inbred lines in wheat. Euphytica 165, 615–624.

    Article  CAS  Google Scholar 

  • Uauy C., Distelfeld A., Fahima T., Blechl A. and Dubcovsky J. 2006 A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314, 1298–1301.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang L., Cui F., Wang L. J., Ding A. M., Zhao C. H., Li X. F. et al. 2012 Conditional QTL mapping of protein content in wheat with respect to grain yield and its components. J. Genet. 91, 303–312.

    Article  PubMed  Google Scholar 

  • Zhang K. P., Chen G. F., Zhao L., Liu B., Xu X. B. and Tian J. C. 2009a Molecular genetic analysis of flour color using a doubled haploid population in bread wheat (Triticum aestivum L.). Euphytica 165, 471–484.

    Article  CAS  Google Scholar 

  • Zhang Y. L., Wu Y. P., Xiao Y. G., He Z. H., Zhang Y., Yan J. et al. 2009b QTL mapping for flour and noodle colour components and yellow pigment content in common wheat. Euphytica 165, 435–444.

    Article  CAS  Google Scholar 

  • Zhao J. L., Chen M. S., Ma Y. M., Li R. J., Ren Y. P., Sun Q. Q. et al. 2009 QTL mapping for quality traits of Chinese dry noodle. Agr. Sci. China 8, 394–400.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by ‘China Agriculture Research System of Wheat’ (CARS-3-03B) and the ‘National Key Technologies R & D Programme’ (grant no. 2011BAD35B03).

Author information

Authors and Affiliations

  1. Tai’an Academy of Agricultural Science, Tai’an, 271000, People’s Republic of China

    XIANYIN SUN, KE WU & ZHAOGUO QIAN

  2. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    XIANYIN SUN, YAN ZHAO, FANMEI KONG, YING GUO & SISHEN LI

  3. Shandong Academy of Agricultural Sciences, Jinan, 250100, People’s Republic of China

    YINGYING WANG

Authors
  1. XIANYIN SUN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. KE WU
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. YAN ZHAO
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ZHAOGUO QIAN
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. FANMEI KONG
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. YING GUO
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. YINGYING WANG
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. SISHEN LI
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to SISHEN LI.

Additional information

[Sun X., Wu K., Zhao Y., Qian Z., Kong F., Guo Y., Wang Y. and Li S. 2016 Molecular genetic analysis of grain protein content and flour whiteness degree using RILs in common wheat. J. Genet. 95, xx–xx]

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

SUN, X., WU, K., ZHAO, Y. et al. Molecular genetic analysis of grain protein content and flour whiteness degree using RILs in common wheat. J Genet 95, 317–324 (2016). https://doi.org/10.1007/s12041-016-0639-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12041-016-0639-9

Keywords

Search

Navigation