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Nuclear genome constitution and other characteristics of somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum

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Abstract

Eleven somatic hybrids (2n = 68 to 74) obtained between S. tuberosum ssp. tuberosum cv. Dejima (2n = 48) and ATDH-1 (2n = 24), an anther-culture-derived dihaploid of S. acaule (Yamada et al., 1997), were characterized by nuclear RFLP markers using 49 single-copy DNA probes distributed throughout the potato genome (2 to 6 probes per chromosome). One of the somatic hybrids, DA8-2, had 72 chromosomes and all the Dejima- and ATDH-1-specific markers (124 and 103 bands, respectively), suggesting the presence of a whole set of both parental chromosomes. The other somatic hybrids lost varying numbers of markers up to seventeen. The pattern of the loss of markers indicated the elimination of five chromosomes among four somatic hybrids. A nucleolar organizer region of chromosome 2 was often eliminated in the somatic hybrids. The somatic hybrids studied here had higher frequencies of multivalent formation than the S. tuberosum parent. They had reasonably good seed set when pollinated with S. tuberosum pollen. Hence, homoeologous recombination between S. acaule and S. tuberosum chromosomes is possible and useful traits from S. acaule may be transferred to the S. tuberosum gene pool.

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Author notes

  1. Tetsuya Yamada

    Present address: Department of Biotechnology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki, 305, Japan

Authors and Affiliations

  1. Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Nada, Kobe, 657, Japan

    Tetsuya Yamada

  2. Experimental Farm, Kobe University, 1348 Uzurano, Kasai, Hyogo, 675-21, Japan

    Kazuyoshi Hosaka

  3. Laboratory of Plant Breeding, Faculty of Agriculture, Kobe University, Nada, Kobe, 657, Japan

    Kumi Nakagawa, Noriyo Kaide, Shuji Misoo & Osamu Kamijima

Authors
  1. Tetsuya Yamada
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  2. Kazuyoshi Hosaka
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  3. Kumi Nakagawa
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  4. Noriyo Kaide
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  5. Shuji Misoo
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  6. Osamu Kamijima
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Yamada, T., Hosaka, K., Nakagawa, K. et al. Nuclear genome constitution and other characteristics of somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum. Euphytica 102, 239–246 (1998). https://doi.org/10.1023/A:1018325529119

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