Skip to main content
Log in

Nucleotide sequence of the potato rDNA intergenic spacer

  • Update Section
  • Short Communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The large intergenic spacer (IGS) of potato rDNA was sequenced and compared to the IGS sequence of tomato. Both spacers exhibit similar length and architecture. Absence of repeated elements down-stream of the putative transcription initiation site (TIS) in potato is compensated by the larger number of subrepeats upstream of the TIS. Especially high level of similarity (86% and 86.5%, respectively) is found in the AT-rich domain containing the TIS and the region approx. 800 bp upstream of the 18S rRNA gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Barker RF, Harberd NP, Jarvis MG, Flavell RB: Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat. J Mol Biol 201: 1–17 (1988).

    Google Scholar 

  2. Bonierbale MW, Plaisted RL, Tanksley SD: RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120: 1095–1103 (1988).

    Google Scholar 

  3. Borisjuk NB, Kostyshin SS, Volkov RA, Miroshnichenko GP: Ribosomal RNA gene organization in higher plant from Nicotiana genus. Mol Biol (Russia). 23: 1067–1074 (1989).

    Google Scholar 

  4. Delcasso-Tremousaygue D, Grellet F, Panabieres F, Ananiev ED, Delseny M: Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant. Eur J Biochem 172: 767–776 (1988).

    Google Scholar 

  5. Gebhardt C, Ritter E, Barone A, Debener T Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F: RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83: 49–57 (1991).

    Google Scholar 

  6. Gründler P, Unfried I, Pascher K, Schweizer D: rDNA intergenic region from Arabidopsis thaliana. Structural analysis, intraspecific variation and functional implications. J Mol Biol 221: 1209–1222 (1991).

    Google Scholar 

  7. Kato A, Nakajima T, Yamashita J, Yakura K, Tanifuji S: The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum. Plant Mol Biol 14: 983–993 (1990).

    Google Scholar 

  8. Kawagoe Y, Kikuta Y: Chloroplast DNA evolution in potato (Solanum tuberosum L.). Theor Appl Genet 81: 13–20 (1991).

    Google Scholar 

  9. Kelly RJ, Siegel A: The Cucurbita maxima ribosomal DNA intergenic spacer has a complex structure. Gene 80: 239–248 (1989).

    Google Scholar 

  10. Kiss T, Kis M, Solymosy F: Nucleotide sequence of a 25S rRNA gene from tomato. Nucl Acids Res 17: 796 (1989).

    Google Scholar 

  11. Kiss T, Szkukalek A, Solymosy F: Nucleotide sequence of a 17S (18S) rRNA gene from tomato. Nucl Acids Res 17: 2127 (1989).

    Google Scholar 

  12. McMullen MD, Hunter B, Phillips R, Rubenstein I: The structure of the maize ribosomal DNA spacer region. Nucl Acids Res 14: 4953–4968 (1986).

    Google Scholar 

  13. Perry KL, Palukaitis P: Transcription of tomato ribosomal DNA and the organization of the intergenic spacer. Mol Gen Genet 221: 102–112 (1990).

    Google Scholar 

  14. Schmidt-Puchta W, Günther I, Sänger HL: Nucleotide sequence of the intergenic spacer (IGS) of the tomato ribosomal DNA. Plant Mol Biol 13: 251–253 (1989).

    Google Scholar 

  15. Takaiwa F, Kikuchi S, Oono K: The complete nucleotide sequence of the intergenic spacer between 25S and 17S rDNAs in rice. Plant Mol Biol 15: 933–935 (1990).

    Google Scholar 

  16. Tremousaygue D, Laudie M, Grellet F, Delseny M: The Brassica oleracea rDNA spacer revisited. Plant Mol Biol 18: 1013–1018 (1992).

    Google Scholar 

  17. Zentgraf U, Ganal M, Hemleben V: Length heterogeneity of the rRNA precursor in cucumber (Cucumis sativus). Plant Mol Biol 15: 465–474 (1990).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borisjuk, N., Hemleben, V. Nucleotide sequence of the potato rDNA intergenic spacer. Plant Mol Biol 21, 381–384 (1993). https://doi.org/10.1007/BF00019953

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00019953

Key words

Navigation